Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065)

Rabbit polyclonal MASH1/Achaete-scute homolog 1 antibody. Validated in WB, IHC, ICC/IF and tested in Mouse, Rat, Human, Zebrafish. Cited in 10 publication(s). Independently reviewed in 5 review(s).

Overview

  • Product name

    Anti-MASH1/Achaete-scute homolog 1 antibody
    See all MASH1/Achaete-scute homolog 1 primary antibodies
  • Description

    Rabbit polyclonal to MASH1/Achaete-scute homolog 1
  • Host species

    Rabbit
  • Specificity

    Ab74065 has been batch tested in WB and IF only. Some customers have successfully used this product in IHC. Please contact Abcam Scientific Support for more information.
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Zebrafish
    Predicted to work with: Chicken, Dog, Xenopus laevis, Rhesus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human MASH1/Achaete-scute homolog 1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab74622.)

  • Positive control

    • This antibody gave a positive signal in the following lysates: DU 145 Whole Cell Rat Brain Tissue Mouse Brain Tissue IF/ICC: SKNSH cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab74065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-P 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Activates transcription by binding to the E box (5'-CANNTG-3').
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Achaete scute complex homolog 1 antibody
    • Achaete scute complex homolog like 1 antibody
    • Achaete scute complex homologue 1 antibody
    • Achaete scute complex homologue like 1 antibody
    • Achaete scute complex like 1 antibody
    • Achaete scute family bHLH transcription factor 1 antibody
    • Achaete scute protein antibody
    • Achaete-scute homolog 1 antibody
    • Ascl 1 antibody
    • ascl1 antibody
    • ASCL1_HUMAN antibody
    • Ash 1 antibody
    • ASH-1 antibody
    • Ash1 antibody
    • bHLHa46 antibody
    • Class A basic helix-loop-helix protein 46 antibody
    • Hash 1 antibody
    • HASH1 antibody
    • Mammalian achaete scute homolog 1 antibody
    • Mammalian achaete scute homologue 1 antibody
    • Mash 1 antibody
    • Mash1 antibody
    see all

Images

  • IHC-P image of MASH1 staining on mouse SVZ sections using ab74065 (1:250). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid pH 6. The sections were then blocked using 1% BSA solution at 21°C for 10 mins. The primary antibiody was incubated at 21°C for 2 hours.

    See Abreview

  • All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065) at 1 µg/ml

    Lane 1 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25,28 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.

  • ICC/IF image of ab74065 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74065, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065) at 1 µg/ml

    Lane 1 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 25 kDa


    Exposure time: 3 minutes
  • All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : Mouse brain homogenate at 20 µg

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 26 kDa why is the actual band size different from the predicted?


    Exposure time: 5 minutes
  • IHC-P image of MASH1 staining on rat SVZ sections using ab74065 (1:1250). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid pH 6. The sections were then blocked using 1% BSA solution at 21°C for 10 mins. The primary antibiody was incubated at 21°C for 2 hours.

    See Abreview

References

This product has been referenced in:

  • Mitra S  et al. Dual regulation of lin28a by Myc is necessary during zebrafish retina regeneration. J Cell Biol 218:489-507 (2019). Read more (PubMed: 30606747) »
  • Mitra S  et al. Histone Deacetylase-Mediated Müller Glia Reprogramming through Her4.1-Lin28a Axis Is Essential for Retina Regeneration in Zebrafish. iScience 7:68-84 (2018). Read more (PubMed: 30267687) »
See all 12 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer - tween 0,05 ph6
Sample
Chicken Tissue sections (Transverse section of chick embryos (spinal cord))
Specification
Transverse section of chick embryos (spinal cord)
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 16 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse brain)
Specification
Mouse brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
(agent) for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Mr. Carl Hobbs

Verified customer

Submitted Oct 03 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Sep 17 2012

Answer

Es freut mich zu hören, dass Sie unser Angebot unseren ab74065 Anti-MASH1/Achaete-scute homolog 1 Antikörper in IHC zu testen, annehmen möchten. Wie besprochen können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Discountcode hat die Nummer 12345678, deckt den Wert von ab74065 ab und kann zum Kauf weiterer Produkte eingesetzt werden.

Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test in IHC eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount

Read More

Answer

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

To our knowledge,ab38557 and ab74065have not been tested in marmosets.

The immunogens for both antibodies show a high homology to Callithrix jacchus:

ab38557 :95%
ab74065: 100%

Therefore, I can offer a discount off a future purchase if you buy ab38557 or ab74065now, test it inmarmosets and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of ab38557 or ab74065 off a future order.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab38557 or ab74065in marmosets. I will then send a discount code. This code must be issued before purchasingab38557 or ab74065 so please wait for my reply before ordering.

2. Purchase ab38557 or ab74065 either by phone, fax, or online (www.abcam.com).

3. Test it in marmosets.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyproduct ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe tested antibodyturns out to be unsuitable for marmosets, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
No
Specification
Brain
Fixative
Paraformaldehyde

Dr. Sophie Pezet

Verified customer

Submitted Jun 08 2012

Question
Answer

DISCOUNT CODE: **********Expiration date: ********* I am very pleased to hear you would like to accept our offer and test ab74065 in ChIP. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for ChIP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

Read More
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde

Dr. Sophie Pezet

Verified customer

Submitted Sep 08 2010

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