Recombinant Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592] (ab213151)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19592] to MASH1/Achaete-scute homolog 1
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19592]
See all MASH1/Achaete-scute homolog 1 primary antibodies -
Description
Rabbit monoclonal [EPR19592] to MASH1/Achaete-scute homolog 1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human medullary thyroid carcinoma and lung small cell carcinoma tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19592 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab213151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
May play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Activates transcription by binding to the E box (5'-CANNTG-3'). -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 429 Human
- Entrez Gene: 17172 Mouse
- Omim: 100790 Human
- SwissProt: P50553 Human
- SwissProt: Q02067 Mouse
- Unigene: 703025 Human
- Unigene: 136217 Mouse
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Alternative names
- Achaete scute complex homolog 1 antibody
- Achaete scute complex homolog like 1 antibody
- Achaete scute complex homologue 1 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human lung small cell carcinoma tissue labeling MASH1/Achaete-scute homolog 1 with ab213151 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Nuclear staining on human lung small cell carcinoma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human medullary thyroid carcinoma tissue labeling MASH1/Achaete-scute homolog 1 with ab213151 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Nuclear staining on human medullary thyroid carcinoma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human lung tissue labeling MASH1/Achaete-scute homolog 1 with ab213151 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Negative staining on human lung. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human thyroid tissue labeling MASH1/Achaete-scute homolog 1 with ab213151 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Negative staining on human thyroid. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue labeling MASH1/Achaete-scute homolog 1 with ab213151 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Negative staining on human lung squamous cell carcinoma.
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue labeling MASH1/Achaete-scute homolog 1 with ab213151 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Negative staining on human lung adenocarcinoma.
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab213151 has been referenced in 2 publications.
- Zhang Z et al. Histone Methylations Define Neural Stem/Progenitor Cell Subtypes in the Mouse Subventricular Zone. Mol Neurobiol 57:997-1008 (2020). PubMed: 31654318
- Sato Y et al. Integrated Immunohistochemical Study on Small-Cell Carcinoma of the Lung Focusing on Transcription and Co-Transcription Factors. Diagnostics (Basel) 10:N/A (2020). PubMed: 33202998