Overview

  • Product name

    Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840]
    See all MASH1/Achaete-scute homolog 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR19840] to MASH1/Achaete-scute homolog 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein within Mouse MASH1/Achaete-scute homolog 1 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q02067

  • Positive control

    • WB: Mouse medullary thyroid cancer lysate; NCI-H69 whole cell lysate. IHC-P: Mouse small cell lung cancer section; Ascl1CreER/+; ROSA26mTmG/+ mouse thyroid tissue. ICC/IF: NCI-H69 cells. IP: Mouse medullary thyroid cancer lysate; NCI-H69 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab211327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).
ICC/IF 1/100.
IP 1/30.

Target

  • Function

    May play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Activates transcription by binding to the E box (5'-CANNTG-3').
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Achaete scute complex homolog 1 antibody
    • Achaete scute complex homolog like 1 antibody
    • Achaete scute complex homologue 1 antibody
    • Achaete scute complex homologue like 1 antibody
    • Achaete scute complex like 1 antibody
    • Achaete scute family bHLH transcription factor 1 antibody
    • Achaete scute protein antibody
    • Achaete-scute homolog 1 antibody
    • Ascl 1 antibody
    • ascl1 antibody
    • ASCL1_HUMAN antibody
    • Ash 1 antibody
    • ASH-1 antibody
    • Ash1 antibody
    • bHLHa46 antibody
    • Class A basic helix-loop-helix protein 46 antibody
    • Hash 1 antibody
    • HASH1 antibody
    • Mammalian achaete scute homolog 1 antibody
    • Mammalian achaete scute homologue 1 antibody
    • Mash 1 antibody
    • Mash1 antibody
    see all

Images

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed mouse small cell lung cancer section labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 dilution, followed by Donkey anti Rabbit Alexa-594 at 1/500 dilution (red). 

    Nuclear counterstain: DAPI (blue).

    Positive staining of MASH1/Achaete-scute homolog 1 on mouse small cell lung cancer.

    The image was kindly provided by our collaborator Dr. Hai Song, Zhejiang University.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed Ascl1CreER/+; ROSA26mTmG/+ mouse thyroid tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 dilution, followed by Tyramide Signal Amplification (red). 

    Counterstain: DAPI (blue) and chick anti-GFP (green).

    ROSA26mTmG/+ mice were bred with Ascl1CreER/+ mice, in which the Ascl1 promoter drives the expression of Cre, to generate mice carrying the genotype of Ascl1CreER/+; ROSA26mTmG/+. Cre activation upon Tamoxifen administration resulted in eGFP expression from the ROSA26mTmG/+ allele. The thyroid tissue from Ascl1CreER/+; ROSA26mTmG/+ mouse was fixed and embedded in paraffin. Paraffin section was stained with rabbit anti-MASH1/Achaete-scute homolog 1 (ab211327) antibody (Red color) and chick anti-GFP antibody (Green color). Tyramide signal amplification was used for high-resolution imaging of low-abundance targets.

    The image was kindly provided by our collaborator Dr. Hai Song, Zhejiang University.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327) at 1/1000 dilution

    Lane 1 : Mouse medullary thyroid cancer lysate at 20 µg
    Lane 2 : NCI-H69 (Human small cell lung cancer cell line) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 25 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 15 seconds; Lane 2: 10 seconds

    Ascl1 is highly expressed in medullary thyroid cancer and small cell lung cancer (PMID: 17090547 and 25267614). Our results showed specific band on mouse medullary thyroid cancer lysate and NCI-H69 (Human small cell lung cancer) lysate. The lysates were kindly provided by our collaborator Dr. Hai Song, Zhejiang University.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized NCI-H69 Human small cell lung cancer cells labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 dilution (red).

    The nuclear counterstain is DAPI (blue).

    Nuclear MASH1/Achaete-scute homolog 1 staining on NCI-H69 cells.

    The images were kindly provided by our collaborator Dr. Hai Song, Zhejiang University.

  • MASH1/Achaete-scute homolog 1 was immunoprecipitated from 0.35 mg of mouse medullary thyroid cancer lysate  with ab211327 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211327 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: Mouse medullary thyroid cancer lysate 10 μg (Input).

    Lane 2: ab211327 IP in mouse medullary thyroid cancer lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211327 in mouse medullary thyroid cancer lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • MASH1/Achaete-scute homolog 1 was immunoprecipitated from 0.35 mg of NCI-H69 (Human small cell lung cancer cell line) whole cell lysate  with ab211327 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211327 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: NCI-H69 whole cell lysate 10 μg (Input).

    Lane 2: ab211327 IP in NCI-H69 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211327 in NCI-H69 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

References

ab211327 has not yet been referenced specifically in any publications.

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