Recombinant Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] - BSA and Azide free (ab240385)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19840] to MASH1/Achaete-scute homolog 1 - BSA and Azide free
- Suitable for: WB, ICC/IF, IP, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] - BSA and Azide free
See all MASH1/Achaete-scute homolog 1 primary antibodies -
Description
Rabbit monoclonal [EPR19840] to MASH1/Achaete-scute homolog 1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab240385 is the carrier-free version of ab211327.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19840 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).
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ICC/IF |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa). |
ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Activates transcription by binding to the E box (5'-CANNTG-3'). -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 429 Human
- Entrez Gene: 17172 Mouse
- Omim: 100790 Human
- SwissProt: P50553 Human
- SwissProt: Q02067 Mouse
- Unigene: 703025 Human
- Unigene: 136217 Mouse
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Alternative names
- Achaete scute complex homolog 1 antibody
- Achaete scute complex homolog like 1 antibody
- Achaete scute complex homologue 1 antibody
see all
Images
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Immunohistochemical analysis of 4% paraformaldehyde-fixed mouse small cell lung cancer section labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 dilution, followed by Donkey anti Rabbit Alexa-594 at 1/500 dilution (red).
Nuclear counterstain: DAPI (blue).
Positive staining of MASH1/Achaete-scute homolog 1 on mouse small cell lung cancer.
The image was kindly provided by our collaborator Dr. Hai Song, Zhejiang University.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211327).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of 4% paraformaldehyde-fixed Ascl1CreER/+; ROSA26mTmG/+ mouse thyroid tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 dilution, followed by Tyramide Signal Amplification (red).
Counterstain: DAPI (blue) and chick anti-GFP (green).
ROSA26mTmG/+ mice were bred with Ascl1CreER/+ mice, in which the Ascl1 promoter drives the expression of Cre, to generate mice carrying the genotype of Ascl1CreER/+; ROSA26mTmG/+. Cre activation upon Tamoxifen administration resulted in eGFP expression from the ROSA26mTmG/+ allele. The thyroid tissue from Ascl1CreER/+; ROSA26mTmG/+ mouse was fixed and embedded in paraffin. Paraffin section was stained with rabbit anti-MASH1/Achaete-scute homolog 1 (ab211327) antibody (Red color) and chick anti-GFP antibody (Green color). Tyramide signal amplification was used for high-resolution imaging of low-abundance targets.
The image was kindly provided by our collaborator Dr. Hai Song, Zhejiang University.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211327).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized NCI-H69 Human small cell lung cancer cells labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 dilution (red).
The nuclear counterstain is DAPI (blue).
Nuclear MASH1/Achaete-scute homolog 1 staining on NCI-H69 cells.
The images were kindly provided by our collaborator Dr. Hai Song, Zhejiang University.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211327).
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MASH1/Achaete-scute homolog 1 was immunoprecipitated from 0.35 mg of mouse medullary thyroid cancer lysate with ab211327 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211327 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse medullary thyroid cancer lysate 10 μg (Input).
Lane 2: ab211327 IP in mouse medullary thyroid cancer lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211327 in mouse medullary thyroid cancer lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211327).
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MASH1/Achaete-scute homolog 1 was immunoprecipitated from 0.35 mg of NCI-H69 (Human small cell lung cancer cell line) whole cell lysate with ab211327 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211327 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: NCI-H69 whole cell lysate 10 μg (Input).
Lane 2: ab211327 IP in NCI-H69 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211327 in NCI-H69 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211327).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab240385 has not yet been referenced specifically in any publications.