Key features and details
- Rabbit polyclonal to MASP1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MASP1 antibody
See all MASP1 primary antibodies
DescriptionRabbit polyclonal to MASP1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment (His-T7-tag) corresponding to Human MASP1 aa 20-447. (Expressed in E.coli).
HTVELNNMFGQIQSPGYPDSYPSDSEVTWNITVPDGFRIKLYFMHFNLES SYLCEYDYVKVETEDQVLATFCGRETTDTEQTPGQEVVLSPGSFMSITFR SDFSNEERFTGFDAHYMAVDVDECKEREDEELSCDHYCHNYIGGYYCSCR FGYILHTDNRTCRVECSDNLFTQRTGVITSPDFPNPYPKSSECLYTIELE EGFMVNLQFEDIFDIEDHPEVPCPYDYIKIKVGPKVLGPFCGEKAPEPIS TQSHSVLILFHSDNSGENRGWRLSYRAAGNECPELQPPVHGKIEPSQAKY FFKDQVLVSCDTGYKVLKDNVEMDTFQIECLKDGTWSNKIPTCKIVDCRA PGELEHGLITFSTRNNLTTYKSEIKYSCQEPYYKMLNNNTGIYTCSAQGV WMNKVLGRSLPTCLPVCGLPKFSRKLMA
Database link: P48740
- IHC-P: Human bile duct cancer, skin cancer, kidney, colorectal cancer and lung cancer tissues. WB: HeLa cell lysate; Mouse liver lysate; Recombinant human MASP1 protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232945 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 79 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
RelevanceMASP is a component of the bactericidal ra-reactive factor rarf which specifically binds to RA and R2 polysaccharides expressed by certain enterobacteria. It triggers the activation of complement cascade by activating the C4 and C2 components. It activates the C4 component by cleaving the alpha-chain of C4. The alternative-splicing product from MASP1 gene is called MASP3. MASP1 is associated with smaller MBL oligomers whereas MASP3 is found on larger oligomers. The substrate of MASP3 is unknown.
- Complement factor MASP-3 antibody
- Complement factor MASP3 antibody
- Complement-activating component of Ra-reactive factor antibody
Paraffin-embedded human bile duct cancer tissue stained for MASP1 using ab232945 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human skin cancer tissue stained for MASP1 using ab232945 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human kidney tissue stained for MASP1 using ab232945 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human colorectal cancer tissue stained for MASP1 using ab232945 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human lung cancer tissue stained for MASP1 using ab232945 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-MASP1 antibody (ab232945) at 3 µg/ml + Recombinant human MASP1 protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 79 kDa
All lanes : Anti-MASP1 antibody (ab232945) at 3 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Mouse liver lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 79 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232945 has not yet been referenced specifically in any publications.