Overview

  • Product name

  • Description

    Rabbit polyclonal to MASP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human MASP1.

  • Positive control

    • Recombinant Human MASP3, Catalytic domain (aa 450-721).
  • General notes

    This product was previously named "Anti-MASP3 antibody".

    MASP1 is an isoform of MASP3. As MASP1 is more commonly used in scientific literature, this product is named after it. However, this antibody specifically detect MASP3.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 50% Glycerol, 2.9% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Detects a band of approximately 45 kDa.



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      MASP is a component of the bactericidal ra-reactive factor rarf which specifically binds to RA and R2 polysaccharides expressed by certain enterobacteria. It triggers the activation of complement cascade by activating the C4 and C2 components. It activates the C4 component by cleaving the alpha-chain of C4. The alternative-splicing product from MASP1 gene is called MASP3. MASP1 is associated with smaller MBL oligomers whereas MASP3 is found on larger oligomers. The substrate of MASP3 is unknown.
    • Cellular localization

      Secreted
    • Database links

    • Alternative names

      • Complement factor MASP-3 antibody
      • Complement factor MASP3 antibody
      • Complement-activating component of Ra-reactive factor antibody
      • CRARF antibody
      • Mannose binding lectin associated serine protease 1 antibody
      • Mannose binding protein associated serine protease antibody
      • Mannose-binding lectin-associated serine protease 1 antibody
      • Mannose-binding lectin-associated serine protease-1 antibody
      • MASP antibody
      • PRSS 5 antibody
      • Ra-reactive factor serine protease p100 antibody
      • RaRF antibody
      • Serine protease 5 antibody
      see all

    Images

    • All lanes : Anti-MASP1 antibody (ab65900) at 1/1000 dilution

      Lane 1 : Molecular weight markers
      Lane 2 : 80ng Recombinant Human MASP1 Catalytic Domain (aa450-721)
      Lane 3 : 40ng Recombinant Human MASP1 Catalytic Domain (aa450-721)
      Lane 4 : 20ng Recombinant Human MASP1 Catalytic Domain (aa450-721)
      Lane 5 : 10ng Recombinant Human MASP1 Catalytic Domain (aa450-721)
      Lane 6 : 5ng Recombinant Human MASP1 Catalytic Domain (aa450-721)

      Observed band size: 45 kDa
      why is the actual band size different from the predicted?



      Recombinant Human MASP1 Catalytic Domain (aa450-721) loaded at 4.0, 2.0, 1.0, 0.5 and 0.25ng/µl.

    References

    ab65900 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    5 µg
    Specification
    liver
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Aug 19 2016

    Abreviews
    Application
    Western blot
    Sample
    Human Serum (human and murein serum)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    8 µg
    Specification
    human and murein serum
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Aug 19 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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