RecombinantRabMAb

Recombinant Anti-MASP1 antibody [EPR22680-36] (ab255814)

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Western blot - Anti-MASP1 antibody [EPR22680-36] (ab255814)
  • Western blot - Anti-MASP1 antibody [EPR22680-36] (ab255814)
  • Immunocytochemistry/ Immunofluorescence - Anti-MASP1 antibody [EPR22680-36] (ab255814)
  • Flow Cytometry - Anti-MASP1 antibody [EPR22680-36] (ab255814)
  • Anti-MASP1 antibody [EPR22680-36] (ab255814)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22680-36] to MASP1
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-MASP1 antibody [EPR22680-36]
    See all MASP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22680-36] to MASP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat liver, serum, lung and plasma lysate; mouse plasma, liver and serum lysate. ICC/IF: RAW 264.7 cells. Flow Cyt: RAW 264.7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22680-36

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab255814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 79 kDa.
ICC/IF
1/50.
Flow Cyt
1/50.
Notes
WB
1/1000. Predicted molecular weight: 79 kDa.
ICC/IF
1/50.
Flow Cyt
1/50.
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Relevance

    MASP is a component of the bactericidal ra-reactive factor rarf which specifically binds to RA and R2 polysaccharides expressed by certain enterobacteria. It triggers the activation of complement cascade by activating the C4 and C2 components. It activates the C4 component by cleaving the alpha-chain of C4. The alternative-splicing product from MASP1 gene is called MASP3. MASP1 is associated with smaller MBL oligomers whereas MASP3 is found on larger oligomers. The substrate of MASP3 is unknown.

  • Cellular localization

    Secreted

  • Database links

    • Alternative names

      • Complement factor MASP-3 antibody
      • Complement factor MASP3 antibody
      • Complement-activating component of Ra-reactive factor antibody
      • CRARF antibody
      • Mannose binding lectin associated serine protease 1 antibody
      • Mannose binding protein associated serine protease antibody
      • Mannose-binding lectin-associated serine protease 1 antibody
      • Mannose-binding lectin-associated serine protease-1 antibody
      • MASP antibody
      • PRSS 5 antibody
      • Ra-reactive factor serine protease p100 antibody
      • RaRF antibody
      • Serine protease 5 antibody
      see all

    Images

    • Western blot - Anti-MASP1 antibody [EPR22680-36] (ab255814)
      Western blot - Anti-MASP1 antibody [EPR22680-36] (ab255814)
      All lanes : Anti-MASP1 antibody [EPR22680-36] (ab255814) at 1/1000 dilution

      Lane 1 : Rat liver lysate
      Lane 2 : Rat serum lysate
      Lane 3 : Rat lung lysate
      Lane 4 : Rat plasma lysate
      Lane 5 : Mouse plasma lysate
      Lane 6 : Mouse serum lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 79 kDa
      Observed band size: 90, 28 kDa why is the actual band size different from the predicted?



      The molecular weight observed is consistent with what has been described in the literature (PMID: 24472859, 27232184).

      Exposure times: Lanes 1-3: 59 secs; Lane 4: 26 secs; Lanes 5-6: 15 secs.

      Blocking/Diluting buffer: 5% NFDM/TBST.

    • Western blot - Anti-MASP1 antibody [EPR22680-36] (ab255814)
      Western blot - Anti-MASP1 antibody [EPR22680-36] (ab255814)
      All lanes : Anti-MASP1 antibody [EPR22680-36] (ab255814) at 1/1000 dilution

      Lane 1 : Mouse liver lysate
      Lane 2 : Mouse pancreas lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 79 kDa
      Observed band size: 90 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      The molecular weight observed is consistent with what has been described in the literature (PMID: 24472859, 27232184).

      Negative control: Pancreas (based on Expression Atlas, GTEx and HPA databases).

      Blocking/Diluting buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-MASP1 antibody [EPR22680-36] (ab255814)
      Immunocytochemistry/ Immunofluorescence - Anti-MASP1 antibody [EPR22680-36] (ab255814)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling MASP1 with ab255814 at a 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at a 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 cells is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    • Flow Cytometry - Anti-MASP1 antibody [EPR22680-36] (ab255814)
      Flow Cytometry - Anti-MASP1 antibody [EPR22680-36] (ab255814)

      Flow cytometric analysis of RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling MASP1 with ab255814 at a 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

    • Anti-MASP1 antibody [EPR22680-36] (ab255814)
      Anti-MASP1 antibody [EPR22680-36] (ab255814)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab255814? Please let us know so that we can cite the reference in this datasheet.

    ab255814 has not yet been referenced specifically in any publications.

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