Recombinant Anti-MASP1 antibody [EPR22680-36] (ab255814)
![Western blot - Anti-MASP1 antibody [EPR22680-36] (ab255814)](https://www.abcam.com/ps/products/255/ab255814/Images/ab255814-3-anti-masp1-antibody-epr22680-36-western-blot.jpg "Western blot - Anti-MASP1 antibody [EPR22680-36] (ab255814)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22680-36] to MASP1
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MASP1 antibody [EPR22680-36]
See all MASP1 primary antibodies -
Description
Rabbit monoclonal [EPR22680-36] to MASP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat liver, serum, lung and plasma lysate; mouse plasma, liver and serum lysate. ICC/IF: RAW 264.7 cells. Flow Cyt: RAW 264.7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22680-36 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab255814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 79 kDa.
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| ICC/IF |
1/50.
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| Flow Cyt |
1/50.
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| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 79 kDa. |
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ICC/IF
1/50. |
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Flow Cyt
1/50. |
Target
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Relevance
MASP is a component of the bactericidal ra-reactive factor rarf which specifically binds to RA and R2 polysaccharides expressed by certain enterobacteria. It triggers the activation of complement cascade by activating the C4 and C2 components. It activates the C4 component by cleaving the alpha-chain of C4. The alternative-splicing product from MASP1 gene is called MASP3. MASP1 is associated with smaller MBL oligomers whereas MASP3 is found on larger oligomers. The substrate of MASP3 is unknown. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 5648 Human
- Entrez Gene: 17174 Mouse
- Entrez Gene: 64023 Rat
- Omim: 600521 Human
- SwissProt: P48740 Human
- SwissProt: P98064 Mouse
- SwissProt: Q8CHN8 Rat
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Alternative names
- Complement factor MASP-3 antibody
- Complement factor MASP3 antibody
- Complement-activating component of Ra-reactive factor antibody
see all
Images
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Western blot - Anti-MASP1 antibody [EPR22680-36] (ab255814)All lanes : Anti-MASP1 antibody [EPR22680-36] (ab255814) at 1/1000 dilution
Lane 1 : Rat liver lysate
Lane 2 : Rat serum lysate
Lane 3 : Rat lung lysate
Lane 4 : Rat plasma lysate
Lane 5 : Mouse plasma lysate
Lane 6 : Mouse serum lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 79 kDa
Observed band size: 90, 28 kDa why is the actual band size different from the predicted?The molecular weight observed is consistent with what has been described in the literature (PMID: 24472859, 27232184).
Exposure times: Lanes 1-3: 59 secs; Lane 4: 26 secs; Lanes 5-6: 15 secs.
Blocking/Diluting buffer: 5% NFDM/TBST.
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![Western blot - Anti-MASP1 antibody [EPR22680-36] (ab255814)](https://www.abcam.com/ps/products/255/ab255814/Images/ab255814-4-anti-masp1-antibody-epr22680-36-western-blot-mouse.jpg)
Western blot - Anti-MASP1 antibody [EPR22680-36] (ab255814)All lanes : Anti-MASP1 antibody [EPR22680-36] (ab255814) at 1/1000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Mouse pancreas lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 79 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsThe molecular weight observed is consistent with what has been described in the literature (PMID: 24472859, 27232184).
Negative control: Pancreas (based on Expression Atlas, GTEx and HPA databases).
Blocking/Diluting buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-MASP1 antibody [EPR22680-36] (ab255814)](https://www.abcam.com/ps/products/255/ab255814/Images/ab255814-2-anti-masp1-antibody-epr22680-36-immunocytochemistry-raw-2647-mouse.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MASP1 antibody [EPR22680-36] (ab255814)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling MASP1 with ab255814 at a 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at a 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 cells is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
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![Flow Cytometry - Anti-MASP1 antibody [EPR22680-36] (ab255814)](https://www.abcam.com/ps/products/255/ab255814/Images/ab255814-1-anti-masp1-antibody-epr22680-36-flowcytometry-raw-2647-mouse.jpg)
Flow Cytometry - Anti-MASP1 antibody [EPR22680-36] (ab255814)Flow cytometric analysis of RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling MASP1 with ab255814 at a 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
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![Anti-MASP1 antibody [EPR22680-36] (ab255814)](https://www.abcam.com/ps/products/255/ab255814/Images/ab255814-6-benefits-of-recombinant-antibodies.png)
Anti-MASP1 antibody [EPR22680-36] (ab255814)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab255814 has not yet been referenced specifically in any publications.
