Key features and details
- Rabbit polyclonal to MASPIN
- Suitable for: ELISA, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MASPIN antibody
See all MASPIN primary antibodies
DescriptionRabbit polyclonal to MASPIN
SpecificityThis antibody detects endogenous levels of total MASPIN protein.
Tested applicationsSuitable for: ELISA, ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
A synthesized peptide derived from an internal region of human MASPIN
- Extracts from Hela cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab65136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).|
FunctionTumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Tissue specificityNormal mammary epithelial cells.
Sequence similaritiesBelongs to the serpin family. Ov-serpin subfamily.
Cellular localizationSecreted > extracellular space.
- Information by UniProt
- Maspin antibody
- Ovalbumin antibody
- Peptidase inhibitor 5 antibody
All lanes : Anti-MASPIN antibody (ab65136) at 1/500 dilution
Lane 1 : Extracts from Hela cells
Lane 2 : Extracts from Hela cells, plus immunising peptide
Predicted band size: 42 kDa
Observed band size: 42 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein.The amount of the peptide for the WB is 5-10 ug.
ICC/IF image of ab65136 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65136, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab65136 has been referenced in 1 publication.
- Todd AG et al. SMN, Gemin2 and Gemin3 associate with beta-actin mRNA in the cytoplasm of neuronal cells in vitro. J Mol Biol 401:681-9 (2010). PubMed: 20620147