Anti-Mast Cell Chymase antibody (ab111239)
Key features and details
- Goat polyclonal to Mast Cell Chymase
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Mast Cell Chymase antibody
See all Mast Cell Chymase primary antibodies -
Description
Goat polyclonal to Mast Cell Chymase -
Host species
Goat -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Mast Cell Chymase aa 50-150 (internal sequence) (Cysteine residue).
-
Positive control
- Human testis and tonsil tissue
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 1.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
IHC-P
Use a concentration of 1.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. -
Tissue specificity
Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions. -
Sequence similarities
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. Cytoplasmic granule. Mast cell granules. - Information by UniProt
-
Database links
- Entrez Gene: 1215 Human
- Omim: 118938 Human
- SwissProt: P23946 Human
- Unigene: 135626 Human
-
Alternative names
- Alpha-chymase antibody
- Chymase 1 antibody
- Chymase 1 mast cell antibody
see all
Images
-
ab111239, at 1.5 µg/ml, staining Mast Cell Chymase in formalin fixed, paraffin embedded Human testis tissue by Immunohistochemistry. Biotinylated secondary antibody, followed by alkaline phosphatase streptavidin and chromogen were used to develop staining.
-
ab111239, at 1.5 µg/ml, staining Mast Cell Chymase in formalin fixed, paraffin embedded Human tonsil tissue by Immunohistochemistry. Biotinylated secondary antibody, followed by alkaline phosphatase streptavidin and chromogen were used to develop staining.
Datasheets and documents
-
Datasheet download
References (7)
ab111239 has been referenced in 7 publications.
- Yamanaka-Takaichi M et al. Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa. Int J Mol Sci 22:N/A (2021). PubMed: 33803422
- Kovacs M et al. The pathogenic role of c-Kit+ mast cells in the spinal motor neuron-vascular niche in ALS. Acta Neuropathol Commun 9:136 (2021). PubMed: 34389060
- Trias E et al. Schwann cells orchestrate peripheral nerve inflammation through the expression of CSF1, IL-34, and SCF in amyotrophic lateral sclerosis. Glia 68:1165-1181 (2020). PubMed: 31859421
- Valero-Jiménez A et al. Transmembrane protease, serine 4 (TMPRSS4) is upregulated in IPF lungs and increases the fibrotic response in bleomycin-induced lung injury. PLoS One 13:e0192963 (2018). PubMed: 29529050
- Trias E et al. Mast cells and neutrophils mediate peripheral motor pathway degeneration in ALS. JCI Insight 3:N/A (2018). PubMed: 30282815
- Judd LM et al. Elevated IL-33 expression is associated with pediatric eosinophilic esophagitis, and exogenous IL-33 promotes eosinophilic esophagitis development in mice. Am J Physiol Gastrointest Liver Physiol 310:G13-25 (2016). Mouse . PubMed: 26514775
- Buzzelli JN et al. IL33 Is a Stomach Alarmin That Initiates a Skewed Th2 Response to Injury and Infection. Cell Mol Gastroenterol Hepatol 1:203-221.e3 (2015). PubMed: 28210674