Anti-Mast Cell Chymase antibody [CC1] (ab2377)
- Datasheet
- Specific References (18)
- Protocols
Overview
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Product nameAnti-Mast Cell Chymase antibody [CC1]
See all Mast Cell Chymase primary antibodies -
DescriptionMouse monoclonal [CC1] to Mast Cell Chymase
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Host speciesMouse
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SpecificityAb2377 recognises recombinant human mast cell chymase. However, endogenous protein is not detected in WB due to low expression, therefore we do not guarantee this antibody in WB.
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Tested applicationsSuitable for: IHC-P, IHC-Fr, Flow Cytmore details
Unsuitable for: WB -
Species reactivityReacts with: Rat, Human, Pig
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Immunogen
Purified human skin Chymase.
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Positive control
- Tonsil. IHC-P: FFPE human tonsil tissue sections.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Mast cells contain a number of preformed chemicals mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Chymase is considered to be an important marker of mast cells as well as an important mediator of inflammation.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
PurityProtein G purified
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Primary antibody notesMast cells contain a number of preformed chemicals mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Chymase is considered to be an important marker of mast cells as well as an important mediator of inflammation.
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ClonalityMonoclonal
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Clone numberCC1
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Myelomaunknown
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IsotypeIgG1
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Light chain typeunknown
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
Applications
Our Abpromise guarantee covers the use of ab2377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 0.2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IHC-Fr | Use at an assay dependent concentration. 1/100-1/200 in an ABC method. | |
| Flow Cyt | Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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FunctionMajor secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
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Tissue specificityMast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.
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Sequence similaritiesBelongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
Cellular localizationSecreted. Cytoplasmic granule. Mast cell granules.
- Information by UniProt
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Database links
- Entrez Gene: 1215 Human
- Entrez Gene: 25627 Rat
- Omim: 118938 Human
- SwissProt: P23946 Human
- SwissProt: P50339 Rat
- Unigene: 135626 Human
- Unigene: 10182 Rat
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Alternative names
- Alpha-chymase antibody
- Chymase 1 antibody
- Chymase 1 mast cell antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377)](https://www.abcam.com/ps/products/2/ab2377/Images/ab2377-266736-ap2189339assy49979edit.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377)IHC image of Mast Cell Chymase staining in human tonsil formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2377, 0.2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - Anti-Mast Cell Chymase antibody [CC1] (ab2377)](https://www.abcam.com/ps/products/2/ab2377/Images/ab2377-266737-ID1076ab2377AP218933930secDATASHEETIMAGE.jpg)
Western blot - Anti-Mast Cell Chymase antibody [CC1] (ab2377)All lanes : Anti-Mast Cell Chymase antibody [CC1] (ab2377) at 1 µg/ml
Lane 1 : Recombinant Human Chymase Protein
Lane 2 :Recombinant Human Mast Cell Tryptase protein (denatured) (ab134529)
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 35 kDa (why is the actual band size different from the predicted?)
Exposure time: 30 secondsAb2377 recognises recombinant human mast cell chymase. However, endogenous protein is not detected in WB due to low expression, therefore we do not guarantee this antibody in WB.
References
This product has been referenced in:
- Zheng J et al. Chymase Mediates Injury and Mitochondrial Damage in Cardiomyocytes during Acute Ischemia/Reperfusion in the Dog. PLoS One 9:e94732 (2014). ICC ; Mouse . Read more (PubMed: 24733352) »
- Yadav A et al. Altered immunohistochemical expression of mast cell tryptase and chymase in the pathogenesis of oral submucous fibrosis and malignant transformation of the overlying epithelium. PLoS One 9:e98719 (2014). IHC-P ; Human . Read more (PubMed: 24874976) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"