Anti-Mast Cell Chymase antibody [CC1] (ab2377)

You are very welcome.

Please let us know if there are ever any other ways in which Abcam might help you.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number XXXX. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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The immunogen sequence of antibody ab2377 is Purified human skin Chymase, ab111239, Human testis and tonsil tissue and ab54176 is Purified human skin chymase.

We unfortunately do not have any extra immunogen information other than this. We also haven't
tested these antibodies with rat sample so can't confirm whether these abs will be suitable. The end users have to try for cross reactivity check by themselves. If you are interested in testing, I am happy offering you a 100% Abreview discount code which will entitles you to purchase one antibody free of charge, if you buy ab111239 and submit a RAT specific Abreview.

Full terms and conditions can be found at the following link.

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Vielen Dank für Ihre Anfrage.

Es freut mich sehr zu hören, dass ab2377 gute Ergebnisse liefert.

Ich persönlich habe leider keine Erfahrung mit Sirius Red. Meine Kollegen allerdings haben Sirius Red schon verwendet. Allerding nur als Einzelfärbung in aufeinanderfolgenden Schnitten, wobei die nächsten Schnitte dann die Immunofärbung enthielten.

Vielleicht helfen Ihnen die folgenden Websites und Publikationen bezüglich der Doppel färbung weiter:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1867025/

http://ndt.oxfordjournals.org/content/early/2012/02/01/ndt.gfr671.abstract

http://www.ihcworld.com/_protocols/special_stains/sirius_red.htm

http://stainsfile.info/StainsFile/dyes/35780.htm

http://www.nottingham.ac.uk/pathology/protocols/epsr.html

Es tut mir leid, dass ich Ihnen diesmal nicht wirklich weiterhelfen konnte und wünsche Ihnen viel Erfolg mit Ihren Versuchen.

Vielen Dank für Ihre Anfrage.

Die Abbildung von ab54176 wurde mit einer Gegenfärbung mit Eosin (alleine) gefärbt.

Während die Abbildung von ab111239 eine Gegenfärbung mit Hematoxlin zeigt.

Beide können Sieauch mit ab2377 einsetzen.

Bitte lassen Sie mich wissen, falls ich Ihre Frage falschverstanden habe.

Ich wünsche Ihnen viel Erfolg bei Ihnen Experimenten und verbleibe

Thank you for your phone call.

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab2377 with dog samples. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for dog samplesand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Thank you very much for your interest in ab2377. To our knowledge, ab2377 has not been tested in mouse and rat. Therefore, I can offer a discount off a future purchase if you buy ab2377 now, test it in mouse and rat and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab2377 in mouse and rat. I will then send a discount code. This code must be issued before purchasing ab2377 so please wait for my reply before ordering. DISCOUNT CODE: xxx Expiration date: xxx 2. Purchase ab2377 either by phone, fax, or online (www.abcam.com). 3. Test it in mouse and rat. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab2377 turns out to be unsuitable for mouse and rat, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.