Overview

  • Product name
    Anti-Mast Cell Chymase antibody [CC1]
    See all Mast Cell Chymase primary antibodies
  • Description
    Mouse monoclonal [CC1] to Mast Cell Chymase
  • Host species
    Mouse
  • Specificity
    Ab2377 recognises recombinant human mast cell chymase. However, endogenous protein is not detected in WB due to low expression, therefore we do not guarantee this antibody in WB.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, Flow Cytmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Rat, Human, Pig
  • Immunogen

    Purified human skin Chymase.

  • Positive control
    • Tonsil. IHC-P: FFPE human tonsil tissue sections.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Mast cells contain a number of preformed chemicals mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Chymase is considered to be an important marker of mast cells as well as an important mediator of inflammation.

Properties

Applications

Our Abpromise guarantee covers the use of ab2377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration. 1/100-1/200 in an ABC method.
Flow Cyt Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
    • Tissue specificity
      Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.
    • Sequence similarities
      Belongs to the peptidase S1 family. Granzyme subfamily.
      Contains 1 peptidase S1 domain.
    • Cellular localization
      Secreted. Cytoplasmic granule. Mast cell granules.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha-chymase antibody
      • Chymase 1 antibody
      • Chymase 1 mast cell antibody
      • chymase 1 preproprotein transcript E antibody
      • chymase 1 preproprotein transcript I antibody
      • Chymase antibody
      • Chymase, heart antibody
      • Chymase, mast cell antibody
      • CMA1 antibody
      • CMA1_HUMAN antibody
      • CYH antibody
      • CYM antibody
      • EC 3.4.21.39 antibody
      • Mast cell chymase 1 antibody
      • Mast cell protease 3 antibody
      • Mast cell protease 5 antibody
      • Mast cell protease I antibody
      • Mast cell protease III antibody
      • Mcp-5 antibody
      • MCP3P antibody
      • Mcpt5 antibody
      • MCT1 antibody
      • MGC119890 antibody
      • MGC119891 antibody
      • MMCP-5 antibody
      see all

    Images

    • IHC image of Mast Cell Chymase staining in human tonsil formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2377, 0.2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • All lanes : Anti-Mast Cell Chymase antibody [CC1] (ab2377) at 1 µg/ml

      Lane 1 : Recombinant Human Chymase Protein
      Lane 2 : Recombinant Human Mast Cell Tryptase protein (denatured) (ab134529)

      Lysates/proteins at 0.1 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 27 kDa
      Observed band size: 35 kDa
      why is the actual band size different from the predicted?


      Exposure time: 30 seconds


       

       

      Ab2377 recognises recombinant human mast cell chymase. However, endogenous protein is not detected in WB due to low expression, therefore we do not guarantee this antibody in WB.

    References

    This product has been referenced in:
    See all 21 Publications for this product

    Customer reviews and Q&As

    1-10 of 20 Abreviews or Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Aug 15 2016

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
    Sample
    Human Cell (Mast cell)
    Specification
    Mast cell
    Permeabilization
    No
    Fixative
    BD Biosciences fixation/permeabilization solution

    Abcam user community

    Verified customer

    Submitted Aug 05 2014

    Question
    Answer

    You are very welcome.

    Please let us know if there are ever any other ways in which Abcam might help you.

    Read More

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number XXXX. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

    Read More

    Answer

    Thank you for contacting us.

    The immunogen sequence of antibody ab2377 is Purified human skin Chymase, ab111239, Human testis and tonsil tissue and ab54176 is Purified human skin chymase.

    We unfortunately do not have any extra immunogen information other than this. We also haven't
    tested these antibodies with rat sample so can't confirm whether these abs will be suitable. The end users have to try for cross reactivity check by themselves. If you are interested in testing, I am happy offering you a 100% Abreview discount code which will entitles you to purchase one antibody free of charge, if you buy ab111239 and submit a RAT specific Abreview.

    Full terms and conditions can be found at the following link.

    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Answer

    Vielen Dank für Ihre Anfrage.

    Es freut mich sehr zu hören, dass ab2377 gute Ergebnisse liefert.

    Ich persönlich habe leider keine Erfahrung mit Sirius Red. Meine Kollegen allerdings haben Sirius Red schon verwendet. Allerding nur als Einzelfärbung in aufeinanderfolgenden Schnitten, wobei die nächsten Schnitte dann die Immunofärbung enthielten.

    Vielleicht helfen Ihnen die folgenden Websites und Publikationen bezüglich der Doppel färbung weiter:

    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1867025/

    http://ndt.oxfordjournals.org/content/early/2012/02/01/ndt.gfr671.abstract

    http://www.ihcworld.com/_protocols/special_stains/sirius_red.htm

    http://stainsfile.info/StainsFile/dyes/35780.htm

    http://www.nottingham.ac.uk/pathology/protocols/epsr.html

    Es tut mir leid, dass ich Ihnen diesmal nicht wirklich weiterhelfen konnte und wünsche Ihnen viel Erfolg mit Ihren Versuchen.

    Read More

    Answer

    Vielen Dank für Ihre Anfrage.

    Die Abbildung von ab54176 wurde mit einer Gegenfärbung mit Eosin (alleine) gefärbt.

    Während die Abbildung von ab111239 eine Gegenfärbung mit Hematoxlin zeigt.

    Beide können Sieauch mit ab2377 einsetzen.

    Bitte lassen Sie mich wissen, falls ich Ihre Frage falschverstanden habe.

    Ich wünsche Ihnen viel Erfolg bei Ihnen Experimenten und verbleibe

    Read More

    Question
    Answer

    Thank you for your phone call.

    DISCOUNT CODE: xxx
    Expiration date: xxx

    I am very pleased to hear you would like to accept our offer and test ab2377 with dog samples. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for dog samplesand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    Read More

    Answer

    Thank you very much for your interest in ab2377. To our knowledge, ab2377 has not been tested in mouse and rat. Therefore, I can offer a discount off a future purchase if you buy ab2377 now, test it in mouse and rat and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab2377 in mouse and rat. I will then send a discount code. This code must be issued before purchasing ab2377 so please wait for my reply before ordering. DISCOUNT CODE: xxx Expiration date: xxx 2. Purchase ab2377 either by phone, fax, or online (www.abcam.com). 3. Test it in mouse and rat. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab2377 turns out to be unsuitable for mouse and rat, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Kidney)
    Specification
    Kidney
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.3% H2O2
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C

    Prof. Chung-Ming Chen

    Verified customer

    Submitted Jan 09 2009

    1-10 of 20 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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