Recombinant
RabMAb

Recombinant Anti-Mast Cell Chymase antibody [EPR13136] - BSA and Azide free (ab233729)

Overview

  • Product name

    Anti-Mast Cell Chymase antibody [EPR13136] - BSA and Azide free
    See all Mast Cell Chymase primary antibodies
  • Description

    Rabbit monoclonal [EPR13136] to Mast Cell Chymase - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Mast Cell Chymase aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: P23946

  • General notes

    Ab233729 is the carrier-free version of ab186417. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab233729 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.??

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab233729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
  • Tissue specificity

    Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.
  • Sequence similarities

    Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localization

    Secreted. Cytoplasmic granule. Mast cell granules.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha-chymase antibody
    • Chymase 1 antibody
    • Chymase 1 mast cell antibody
    • chymase 1 preproprotein transcript E antibody
    • chymase 1 preproprotein transcript I antibody
    • Chymase antibody
    • Chymase, heart antibody
    • Chymase, mast cell antibody
    • CMA1 antibody
    • CMA1_HUMAN antibody
    • CYH antibody
    • CYM antibody
    • EC 3.4.21.39 antibody
    • Mast cell chymase 1 antibody
    • Mast cell protease 3 antibody
    • Mast cell protease 5 antibody
    • Mast cell protease I antibody
    • Mast cell protease III antibody
    • Mcp-5 antibody
    • MCP3P antibody
    • Mcpt5 antibody
    • MCT1 antibody
    • MGC119890 antibody
    • MGC119891 antibody
    • MMCP-5 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human colon tissue labeling Mast Cell Chymase with ab186417 at 1/500 dilution. Secondary staining with HRP Polymer for Rabbit IgG and counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186417).

References

ab233729 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab233729.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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