Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13136] to Mast Cell Chymase - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Product nameAnti-Mast Cell Chymase antibody [EPR13136] - BSA and Azide free
See all Mast Cell Chymase primary antibodies
DescriptionRabbit monoclonal [EPR13136] to Mast Cell Chymase - BSA and Azide free
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human Mast Cell Chymase aa 200 to the C-terminus. The exact sequence is proprietary.
Database link: P23946
Ab233729 is the carrier-free version of ab186417. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab233729 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab233729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionMajor secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
Tissue specificityMast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.
Sequence similaritiesBelongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain.
Cellular localizationSecreted. Cytoplasmic granule. Mast cell granules.
- Information by UniProt
- Alpha-chymase antibody
- Chymase 1 antibody
- Chymase 1 mast cell antibody
Immunohistochemical analysis of paraffin embedded Human colon tissue labeling Mast Cell Chymase with ab186417 at 1/500 dilution. Secondary staining with HRP Polymer for Rabbit IgG and counterstained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186417).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233729 has not yet been referenced specifically in any publications.