Application
Western blot
Sample
Human Tissue lysate - whole (healthy skin and skin with mastocytosis)
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-16% gel)
Loading amount
20 µg
Specification
healthy skin and skin with mastocytosis
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Dilution
1/10000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: goat anti rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Chemiluminescence
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 27 kDa
Abcam user community
Verified customer
Submitted Nov 19 2019