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    mast-cell-tryptase-antibody-aa1-ab2378.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-Mast Cell Tryptase antibody [AA1] (ab2378)

  • Datasheet
  • SDS
Reviews (35)Q&A (20)References (101)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
  • Western blot - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)

Key features and details

  • Mouse monoclonal [AA1] to Mast Cell Tryptase
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Mast Cell Tryptase antibody [AA1]
    See all Mast Cell Tryptase primary antibodies
  • Description

    Mouse monoclonal [AA1] to Mast Cell Tryptase
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cat, Dog, Monkey
  • Immunogen

    Full length native protein (purified) corresponding to Human Mast Cell Tryptase. Human mast cell tryptase was purified from lung tissue by high salt extraction, ammonium sulphate precipitation, octyl Sepharose and heparin-agarose chromatography. (PMID 2253091)

  • Positive control

    • WB: Human lung, tonsil and skin tissue lysate. IHC-P: Human skin tissue.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    AA1
  • Myeloma

    unknown
  • Isotype

    IgG1
  • Light chain type

    unknown
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human Mast Cell Tryptase protein (denatured) (ab134529)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (4)
1/100 - 1/500. Detects a band of approximately 35-37 kDa (predicted molecular weight: 31 kDa).

We recommend using 3% milk as the blocking agent for Western blot.

IHC-P (19)
1/200000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/100 - 1/500. Detects a band of approximately 35-37 kDa (predicted molecular weight: 31 kDa).

We recommend using 3% milk as the blocking agent for Western blot.

IHC-P
1/200000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation.
  • Cellular localization

    Secreted. Note: released from the secretory granules upon mast cell activation.
  • Database links

    • Entrez Gene: 7177 Human
    • Entrez Gene: 100503895 Mouse
    • Entrez Gene: 54271 Rat
    • Omim: 191080 Human
    • SwissProt: Q15661 Human
    • SwissProt: Q02844 Mouse
    • SwissProt: P27435 Rat
    • Alternative names

      • alpha II antibody
      • Lung tryptase antibody
      • Mast cell alpha II tryptase antibody
      • Mast cell beta I tryptase antibody
      • Mast cell protease 7 antibody
      • Mast cell protease II antibody
      • MCP 7 antibody
      • Pituitary tryptase antibody
      • Skin tryptase antibody
      • TPS 1 antibody
      • TPS1 antibody
      • TPS2 antibody
      • TPSAB1 antibody
      • TPSAB1 protein antibody
      • TPSB1 antibody
      • Tryptase 1 antibody
      • Tryptase alpha 1 antibody
      • tryptase alpha I included antibody
      • Tryptase alpha II antibody
      • tryptase alpha II included antibody
      • tryptase alpha included antibody
      • tryptase alpha/beta 1 antibody
      • Tryptase beta 1 antibody
      • tryptase beta I included antibody
      • Tryptase I antibody
      • tryptase I included antibody
      • Tryptase III antibody
      • Tryptase skin antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)

      IHC image of Mast Cell Tryptase staining in Human Normal Skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. The section was then incubated with ab2378, 1/200,000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Western blot - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
      Western blot - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
      Lanes 1-3 : Anti-Mast Cell Tryptase antibody [AA1] (ab2378) at 1/100 dilution
      Lanes 4-6 : Anti-Mast Cell Tryptase antibody [AA1] (ab2378) at 1/500 dilution

      Lanes 1 & 4 : Human lung normal tissue lysate - total protein (40 - 65 years)
      Lanes 2 & 5 : Human tonsil normal tissue lysate - total protein
      Lanes 3 & 6 : Human skin tissue lysate - total protein

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 31 kDa


      Exposure time: 2 minutes


      Mast Cell Tryptase contains an 18-residue signal sequence and a 12-residue activation peptide. The protein also has two potential glycosylation sites (Swissprot data). These post-translational modifications might explain the banding pattern observed. Abcam used 5% milk in TBS-T as a blocking agent for this blot. We found that this blocking agent removed more non-specific bands than BSA.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)Meyer at al PLoS One. 2013;8(2):e56773. doi: 10.1371/journal.pone.0056773. Epub 2013 Feb 22. Fig 2. Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/

      Tryptase-stained skin biopsy.

      Skin biopsy in CUrt patient stained for tryptase (ab2378, red) at baseline at low (10×, upper panels) and high (60×, lower panels) magnification and at 15 minutes following cold stimulation time test.

      Skin biopsies were obtained at baseline and 15 minutes after CSTT (5 min) on the challenge site, placed into 10% neutral buffered formalin, embedded in paraffin and cut into 5-µm-thick sections. Local anesthesia was performed just before biopsy and did not delay the timing of the biopsy. Slides for immunohistochemistry were deparaffinized and stained. ab2378 was diluted 1:100 and incubated for 2 hours. A biotinylated undiluted secondary antibody of Goat anti-Mouse SS Link, was incubated for 32 minutes, followed with enzyme conjugate, and Fast Red chromogen.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)This image is courtesy of an Abreview submitted by Dr Tom Donndelinger

      ab2378 staining formalin fixed paraffin-embedded human colon tissue sections cut at 1 micron.

      The section was subjected to heat mediated antigen retrieval and permeabilized in Triton-X prior to blocking in 6% BSA for 2 hours at 24°C.  The primary anitbody was diluted 1/250 and incubated with the sample for 16 hours at 5°C.  The secondary antibody was ab7064 (diluted 1/2000) and the slide was counterstained with DAPI.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)De Martin et al PLoS One. 2014 May 15;9(5):e97592. doi: 10.1371/journal.pone.0097592. eCollection 2014. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      AM distribution in thymic mast cells in cryostat sections of human newborn thymic tissue.

      Sections were incubated with an antibody against tryptase (A, B) using ab2378 at a 1/100 dilution.

      V: blood vessel.

      Briefly, frozen sections (10 µm-thick) were cut with a cryostat at −20°C. Antigens were retrieved by heating the sections previously immersed in Bond Epitope Retrieval solution 1 at 100°C for 30 min. Sections were washed in phosphate buffered saline (PBS) and incubated with the primary antibody.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)Image is courtesy of an AbReview submitted by Dr Fancois Daubeuf

      ab2378 staining Mast Cell Tryptase in rat Lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

      Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)Image is courtesy of an AbReview submitted by Carl Hobbs

      ab2378 staining Mast Cell Tryptase in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

      Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/7500 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)Image courtesy of an AbReview submitted by Dr Francois Daubeuf

      ab2378 staining Mast Cell Tryptase in mouse Lung (LPS inflammation) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

      Tissue was fixed with paraformaldehyde and blocked with 4% M.O.M Ig blocking for 1 hour at 20°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/200 in blocking buffer) for 16 hours at 4°C. A Biotin-conjugated Rabbit anti-mouse IgG polyclonal (1/100) was used as the secondary antibody.

      See Abreview

    Protocols

    • Mouse on Mouse staining protocol

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (101)

    Publishing research using ab2378? Please let us know so that we can cite the reference in this datasheet.

    ab2378 has been referenced in 101 publications.

    • Yamanaka-Takaichi M  et al. Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa. Int J Mol Sci 22:N/A (2021). PubMed: 33803422
    • Gunjigake K  et al. Interleukin-17A derived from mast cells contributes to fibrosis in gastric cancer with peritoneal dissemination. Gastric Cancer 24:31-44 (2021). PubMed: 32488650
    • Chen Y  et al. Electroacupuncture at ST36 Relieves Visceral Hypersensitivity via the NGF/TrkA/TRPV1 Peripheral Afferent Pathway in a Rodent Model of Post-Inflammation Rectal Hypersensitivity. J Inflamm Res 14:325-339 (2021). PubMed: 33584100
    • Atiakshin D  et al. Protease profile of normal and neoplastic mast cells in the human bone marrow with special emphasis on systemic mastocytosis. Histochem Cell Biol 155:561-580 (2021). PubMed: 33492488
    • He M  et al. The prognostic significance of tumor-infiltrating lymphocytes in cervical cancer. J Gynecol Oncol 32:e32 (2021). PubMed: 33825354
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Resin sections) abreview for Anti-Mast Cell Tryptase antibody [AA1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Resin sections)
    Sample
    Human Tissue sections (Nasal Polyp and Tonsil)
    Specification
    Nasal Polyp and Tonsil
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 13 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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