Recombinant
RabMAb

Recombinant Anti-Mast Cell Tryptase antibody [EPR8475] - BSA and Azide free (ab227573)

Overview

  • Product name

    Anti-Mast Cell Tryptase antibody [EPR8475] - BSA and Azide free
    See all Mast Cell Tryptase primary antibodies
  • Description

    Rabbit monoclonal [EPR8475] to Mast Cell Tryptase - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: IP or WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Mast Cell Tryptase.

  • Positive control

    • Human colon and tonsil tissues.
  • General notes

    ab227573 is the carrier-free version of ab134931 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab227573 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab227573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For antigen retrieval: heat up to 98 °C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended.

  • Application notes
    Is unsuitable for IP or WB.
  • Target

    • Relevance

      Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation.
    • Cellular localization

      Secreted. Note: released from the secretory granules upon mast cell activation.
    • Database links

    • Alternative names

      • alpha II antibody
      • Lung tryptase antibody
      • Mast cell alpha II tryptase antibody
      • Mast cell beta I tryptase antibody
      • Mast cell protease 7 antibody
      • Mast cell protease II antibody
      • MCP 7 antibody
      • Pituitary tryptase antibody
      • Skin tryptase antibody
      • TPS 1 antibody
      • TPS1 antibody
      • TPS2 antibody
      • TPSAB1 antibody
      • TPSAB1 protein antibody
      • TPSB1 antibody
      • Tryptase 1 antibody
      • Tryptase alpha 1 antibody
      • tryptase alpha I included antibody
      • Tryptase alpha II antibody
      • tryptase alpha II included antibody
      • tryptase alpha included antibody
      • tryptase alpha/beta 1 antibody
      • Tryptase beta 1 antibody
      • tryptase beta I included antibody
      • Tryptase I antibody
      • tryptase I included antibody
      • Tryptase III antibody
      • Tryptase skin antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human colon tissue, labelling Mast Cell Tryptase using ab134931 at a 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134931).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis using ab134931 showing positive staining in Normal stomach tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134931).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis using ab134931 showing positive staining in Colonic adenocarcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134931).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • This IHC data was generated using the same anti-Mast Cell Tryptase antibody clone, EPR8475, in a different buffer formulation (cat# ab134931).

      Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human tonsil tissue, labelling Mast Cell Tryptase using ab134931 at a 1/500 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • This IHC data was generated using the same anti-Mast Cell Tryptase antibody clone, EPR8475, in a different buffer formulation (cat# ab134931).

      Immunohistochemical analysis using ab134931 showing positive staining in Breast carcinoma tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab227573 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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