Recombinant Anti-Mast Cell Tryptase antibody [EPR8476] (ab134932)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8476] (ab134932)](https://www.abcam.com/ps/products/134/ab134932/Images/ab134932-333670-anti-mast-cell-tryptase-antibody-epr8476-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8476] (ab134932)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8476] to Mast Cell Tryptase
- Suitable for: IHC-P
- Reacts with: Human
Overview
-
Product name
Anti-Mast Cell Tryptase antibody [EPR8476]
See all Mast Cell Tryptase primary antibodies -
Description
Rabbit monoclonal [EPR8476] to Mast Cell Tryptase -
Host species
Rabbit -
Specificity
Lab re-tests in mouse and rat species were negative. Therefore, we do not gaurantee this antibody in these species. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to residues in Human Mast Cell Tryptase.
-
Positive control
- IHC-P: Human stomach, colon and tonsil tissues.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8476 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab134932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For antigen retrieval: heat up to 98°C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended. See IHC antigen retrieval protocols. For unpurified use at 1/500 - 1/1000. |
Target
-
Relevance
Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation. -
Cellular localization
Secreted. Note: released from the secretory granules upon mast cell activation. -
Database links
- Entrez Gene: 7177 Human
- Omim: 191080 Human
- SwissProt: Q15661 Human
- Unigene: 405479 Human
-
Alternative names
- alpha II antibody
- Lung tryptase antibody
- Mast cell alpha II tryptase antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8476] (ab134932)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling Mast Cell Tryptase with Purified ab134932 at 1:5000 dilution (0.65 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8476] (ab134932)](https://www.abcam.com/ps/products/134/ab134932/Images/ab134932-169865-anti-mast-cell-tryptase-antibody-epr8476-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8476] (ab134932)This image is courtesy of an anonymous Abreviewab14404 staining Mast Cell Tryptase in Mouse small intestine tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Cells were fixed with paraformaldehyde, blocked with 5% serum for 60 minutes at 21°C and heat mediated antigen retrieval was performed in 10mM Citrate buffer pH6.0. Samples were incubated with primary antibody (1/500 in PBS + 2% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit Ipolyclonal(1/400) was used as the secondary antibody.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8476] (ab134932)](https://www.abcam.com/ps/products/134/ab134932/Images/ab134932-149116-anti-mast-cell-tryptase-antibody-epr8476-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8476] (ab134932)Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human colon tissue, labelling Mast Cell Tryptase using ab134932 at a 1/500 dilution.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8476] (ab134932)](https://www.abcam.com/ps/products/134/ab134932/Images/ab134932-149115-anti-mast-cell-tryptase-antibody-epr8476-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8476] (ab134932)Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human tonsil tissue, labelling Mast Cell Tryptase using ab134932 at a 1/500 dilution.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Anti-Mast Cell Tryptase antibody [EPR8476] (ab134932)](https://www.abcam.com/ps/products/134/ab134932/Images/ab134932-5-benefits-of-recombinant-antibodies.png)
Anti-Mast Cell Tryptase antibody [EPR8476] (ab134932)
Datasheets and documents
References (18)
ab134932 has been referenced in 18 publications.
- Trias E et al. Schwann cells orchestrate peripheral nerve inflammation through the expression of CSF1, IL-34, and SCF in amyotrophic lateral sclerosis. Glia 68:1165-1181 (2020). PubMed: 31859421
- Xiong Y et al. Identification and validation of dichotomous immune subtypes based on intratumoral immune cells infiltration in clear cell renal cell carcinoma patients. J Immunother Cancer 8:N/A (2020). PubMed: 32217761
- Yoshizaki T et al. IL-25 exacerbates autoimmune aortitis in IL-1 receptor antagonist-deficient mice. Sci Rep 9:17067 (2019). PubMed: 31745167
- Suto H et al. IL-25 enhances TH17 cell-mediated contact dermatitis by promoting IL-1ß production by dermal dendritic cells. J Allergy Clin Immunol 142:1500-1509.e10 (2018). PubMed: 29522843
- Huang B et al. Expression of Toll-like receptor 4 on mast cells in gingival tissues of human chronic periodontitis. Mol Med Rep 17:6731-6735 (2018). PubMed: 29488617
