Recombinant Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9522] to Mast Cell Tryptase
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Mast Cell Tryptase antibody [EPR9522]
See all Mast Cell Tryptase primary antibodies -
Description
Rabbit monoclonal [EPR9522] to Mast Cell Tryptase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human Mast Cell Tryptase aa 1-300.
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Positive control
- Human skin, colon cancer or tonsil lysate, Human colon tissue. IP: Human skin lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9522 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
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IHC-P | (4) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
1/50.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 30 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
1/50. |
Target
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Relevance
Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation. -
Cellular localization
Secreted. Note: released from the secretory granules upon mast cell activation. -
Database links
- Entrez Gene: 7177 Human
- Entrez Gene: 100503895 Mouse
- Entrez Gene: 54271 Rat
- Omim: 191080 Human
- SwissProt: Q15661 Human
- SwissProt: Q02844 Mouse
- SwissProt: P27435 Rat
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Alternative names
- alpha II antibody
- Lung tryptase antibody
- Mast cell alpha II tryptase antibody
see all
Images
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All lanes : Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757) at 1/1000 dilution
Lane 1 : Human skin lysate
Lane 2 : Human colon cancer lysate
Lane 3 : Human tonsil lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 30 kDa -
Immunohistochemical analysis of paraffin embedded Human tissue labeling Mast Cell Tryptase with ab151757 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Purified ab151757 at 1/50 dilution (2µg) immunoprecipitating Mast Cell Tryptase in Human skin lysate.
Lane 1 (input): Human skin lysate 10µg
Lane 2 (+): ab151757 + Human skin lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab151757 in Human skin lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 30 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab151757 has been referenced in 11 publications.
- Atiakshin D et al. Protease profile of normal and neoplastic mast cells in the human bone marrow with special emphasis on systemic mastocytosis. Histochem Cell Biol 155:561-580 (2021). PubMed: 33492488
- Janke LJ et al. Development of Mast Cell and Eosinophil Hyperplasia and HLH/MAS-Like Disease in NSG-SGM3 Mice Receiving Human CD34+ Hematopoietic Stem Cells or Patient-Derived Leukemia Xenografts. Vet Pathol 58:181-204 (2021). PubMed: 33208054
- Widner DB et al. Activated mast cells in skeletal muscle can be a potential mediator for cancer-associated cachexia. J Cachexia Sarcopenia Muscle 12:1079-1097 (2021). PubMed: 34008339
- Atiakshin D et al. Expression of CD38 in Mast Cells: Cytological and Histotopographic Features. Cells 10:N/A (2021). PubMed: 34685490
- Orgel K et al. Genetic diversity between mouse strains allows identification of the CC027/GeniUnc strain as an orally reactive model of peanut allergy. J Allergy Clin Immunol 143:1027-1037.e7 (2019). PubMed: 30342892