Recombinant
RabMAb

Recombinant Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)

Overview

  • Product name

    Anti-Mast Cell Tryptase antibody [EPR9522]
    See all Mast Cell Tryptase primary antibodies
  • Description

    Rabbit monoclonal [EPR9522] to Mast Cell Tryptase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Mast Cell Tryptase aa 1-300.

  • Positive control

    • Human skin, colon cancer or tonsil lysate, Human colon tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
IHC-P 1/100 - 1/250.
IP 1/100 - 1/500.

Target

  • Relevance

    Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation.
  • Cellular localization

    Secreted. Note: released from the secretory granules upon mast cell activation.
  • Database links

  • Alternative names

    • alpha II antibody
    • Lung tryptase antibody
    • Mast cell alpha II tryptase antibody
    • Mast cell beta I tryptase antibody
    • Mast cell protease 7 antibody
    • Mast cell protease II antibody
    • MCP 7 antibody
    • Pituitary tryptase antibody
    • Skin tryptase antibody
    • TPS 1 antibody
    • TPS1 antibody
    • TPS2 antibody
    • TPSAB1 antibody
    • TPSAB1 protein antibody
    • TPSB1 antibody
    • Tryptase 1 antibody
    • Tryptase alpha 1 antibody
    • tryptase alpha I included antibody
    • Tryptase alpha II antibody
    • tryptase alpha II included antibody
    • tryptase alpha included antibody
    • tryptase alpha/beta 1 antibody
    • Tryptase beta 1 antibody
    • tryptase beta I included antibody
    • Tryptase I antibody
    • tryptase I included antibody
    • Tryptase III antibody
    • Tryptase skin antibody
    see all

Images

  • All lanes : Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757) at 1/1000 dilution

    Lane 1 : Human skin lysate
    Lane 2 : Human colon cancer lysate
    Lane 3 : Human tonsil lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 30 kDa

  • Immunohistochemical analysis of paraffin embedded Human tissue labeling Mast Cell Tryptase with ab151757 at a 1/100 dilution.

References

This product has been referenced in:

  • Orgel K  et al. Genetic diversity between mouse strains allows identification of the CC027/GeniUnc strain as an orally reactive model of peanut allergy. J Allergy Clin Immunol 143:1027-1037.e7 (2019). Read more (PubMed: 30342892) »
  • Valero-Jiménez A  et al. Transmembrane protease, serine 4 (TMPRSS4) is upregulated in IPF lungs and increases the fibrotic response in bleomycin-induced lung injury. PLoS One 13:e0192963 (2018). Read more (PubMed: 29529050) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Bone marrow)
Antigen retrieval step
Other
Permeabilization
Yes - 0.3% Triton x100
Specification
Bone marrow
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Sun Park

Verified customer

Submitted Oct 25 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Knee)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
No
Specification
Knee
Blocking step
Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
10% NBF fix, 10% Formic Acid decalcification

Premier Laboratory

Verified customer

Submitted May 04 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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