Key features and details
- Rabbit polyclonal to MAST205
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAST205 antibody
See all MAST205 primary antibodies
DescriptionRabbit polyclonal to MAST205
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment corresponding to Human MAST205 aa 512-785. (N-terminal His-tag and GST-tag; Expressed in E.coli).
FETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVER DILTFAENPFVVSMFCSFDTKRHLCMVMEYVEGGDCATLLKNIGALPVDM VRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGL MSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAM GIILYEFLVGCVPFF
Database link: Q6P0Q8-1
- IHC-P: Human testis tissue. WB: Pig cerebrum lysate; Recombinant human MAST205 protein.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab233182 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 196 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionAppears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins. Functions in a multi-protein complex in spermatid maturation. Regulates lipopolysaccharide-induced IL-12 synthesis in macrophages by forming a complex with TRAF6, resulting in the inhibition of TRAF6 NF-kappa-B activation.
Tissue specificityAbundant in the testis.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain.
modificationsPhosphorylated and ubiquitinated. N-terminal ubiquitination leads to degradation of MAST2 by proteasome-mediated proteolysis. N-terminal phosphorylation appears to be a prerequisite for ubiquitination.
Cellular localizationCytoplasm > cytoskeleton. Cell membrane. Recruited to the sub-membranous area on interaction with CDHR2.
- Information by UniProt
- EC 188.8.131.52 3 antibody
- FLJ39200 antibody
- KIAA0807 antibody
Formalin-fixed, paraffin-embedded human testis tissue stained for MAST205 using ab233182 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-MAST205 antibody (ab233182) at 2 µg/ml + Recombinant human MAST205 protein
Predicted band size: 196 kDa
Pig cerebrum lysate
Predicted band size: 196 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233182 has not yet been referenced specifically in any publications.