Product nameAnti-MAST4 antibody
See all MAST4 primary antibodies
DescriptionRabbit polyclonal to MAST4
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human, African green monkey
Predicted to work with: Mouse
Synthetic peptide within Human MAST4 (internal sequence). The exact sequence is proprietary.
Database link: O15021
- Jurkat, COLO, HUVEC and MCF7 cell extracts; Human testis tissue; COS-7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS, 0.87% Sodium chloride
PBS (without Mg2+and Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/500.|
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/3000. Predicted molecular weight: 36 kDa.|
Tissue specificityHighly expressed in most normal human tissues, with an exception of in testis, small intestine, colon and peripheral blood leukocyte.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- MAST4 antibody
- MAST4_HUMAN antibody
- Microtubule associated serine/threonine protein kinase member 4 antibody
- Microtubule-associated serine/threonine-protein kinase 4 antibody
All lanes : Anti-MAST4 antibody (ab196777) at 1/500 dilution
Lane 1 : Jurkat cell extract
Lane 2 : COLO cell extract
Lane 3 : HUVEC cell extract
Lane 4 : MCF7 cell extract
Lane 5 : Jurkat cell extract with synthesized peptide
Predicted band size: 36 kDa
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling MAST4 with ab196777 at 1/50 dilution. The image on the right was treated with synthesized peptide.
Immunofluorescent analysis of COS-7 cells labeling MAST4 with ab196777 at 1/100 dilution. The image on the right was treated with synthesized peptide.
ab196777 has not yet been referenced specifically in any publications.