Key features and details
- Rabbit polyclonal to MAST4
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAST4 antibody
See all MAST4 primary antibodies
DescriptionRabbit polyclonal to MAST4
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
Synthetic peptide corresponding to a region between residue 1900 and 1950 of Human MAST4 (NP_055998.1).
- Whole cell lysate from HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab87734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 266 kDa.|
|IP||Use at 5-10 µg/mg of lysate.|
Tissue specificityHighly expressed in most normal human tissues, with an exception of in testis, small intestine, colon and peripheral blood leukocyte.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- MAST4 antibody
- MAST4_HUMAN antibody
- Microtubule associated serine/threonine protein kinase member 4 antibody
- Microtubule-associated serine/threonine-protein kinase 4 antibody
All lanes : Anti-MAST4 antibody (ab87734) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 266 kDa
Exposure time: 3 minutes
Detection of MAST4 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab87734 at 10 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab87734 has not yet been referenced specifically in any publications.