Recombinant
RabMAb

Recombinant Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846)

Overview

  • Product name

    Anti-MAT1A + MAT2A antibody [EPR19048]
    See all MAT1A + MAT2A primary antibodies
  • Description

    Rabbit monoclonal [EPR19048] to MAT1A + MAT2A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MAT1A + MAT2A aa 50-150. The exact sequence is proprietary.
    Database link: P31153

  • Positive control

    • WB: Ramos, U937, HEK-293T, C6 and NIH/3T3 whole cell lysates; HepG2 untreated and treated with LPS whole cell lysates; Human MAT1A and MAT2A recombinant proteins; Human fetal liver, fetal heart, fetal kidney and fetal spleen lysates; Mouse liver, kidney and spleen lysates; Rat liver, brain and spleen lysates. IP: Ramos whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19048
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab192846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IP 1/30.

Images

  • All lanes : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/20000 dilution

    Lane 1 : Human MAT1A recombinant protein
    Lane 2 : Human MAT2A recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 44,75 kDa
    why is the actual band size different from the predicted?



     

    Exposure times: Lane 1: 1 second; Lane 2: 2 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human MAT1A recombinant protein contains aa1-395 with a His-Tag®. This recombinant protein was made in-house.

  • Lane 1 : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/1000 dilution
    Lanes 2-3 : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/5000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Mouse liver lysate
    Lane 3 : Rat liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
    Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 44 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Lanes 1-3 : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/10000 dilution
    Lanes 4-6 : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/1000 dilution

    Lane 1 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 2 : U937 (human histiocytic lymphoma cell line) whole cell lysate
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 4 : Human fetal heart lysate
    Lane 5 : Human fetal kidney lysate
    Lane 6 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 4-6 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 44 kDa


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/1000 dilution

    Lane 1 : Mouse kidney lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Rat kidney lysate
    Lane 5 : C6 (rat glial tumor cell line) whole cell lysate
    Lane 6 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 44 kDa



     

    Exposure times: Lane 1-2: 5 seconds; Lane 3-6: 3 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • All lanes : Anti-MAT1A + MAT2A antibody [EPR19048] (ab192846) at 1/20000 dilution

    Lane 1 : Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) treated with 500 ng/ml Lipopolysaccharides for 4 hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 44 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile  observed is consistent with what has been described in the literature (PMID: 18695670 and PMID: 22879628).

  • MAT2A was immunoprecipitated from 0.35 mg of Ramos (human Burkitt's lymphoma cell line) whole cell lysate with ab192846 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab192846 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Ramos whole cell lysate 10 µg (Input).

    Lane 2: ab192846 IP in Ramos whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab192846 in Ramos whole cell lysate.

     

    Exposure time: 1 second.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

     

References

ab192846 has not yet been referenced specifically in any publications.

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