Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7938] to MAT1A
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-MAT1A antibody [EPR7938]
See all MAT1A primary antibodies
DescriptionRabbit monoclonal [EPR7938] to MAT1A
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human MAT1A aa 50-150. The exact sequence is proprietary.
- WB: Human fetal liver, Mouse liver, and Rat liver lysates. IP: 293T cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Dissociation constant (KD)KD = 1.22 x 10 -11 M Learn more about KD
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab129176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).|
|IP||1/10 - 1/100.|
RelevanceMAT1A catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. Mutations in this gene are associated with methionine adenosyltransferase deficiency.
- AdoMet antibody
- AdoMet synthetase 1 antibody
- AI046368 antibody
ab129176 (Purified) at 1:40 dilution (2 µg) immunoprecipitating MAT1A in 293T whole cell lysate.
Lane 1 (input): 293T (Human embryonic kidney epithelial cell) whole cell lysate 10 µg
Lane 2 (+): ab129176 & 293T whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129176 in 293T whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes : Anti-MAT1A antibody [EPR7938] (ab129176) at 1/10000 dilution (Purified)
Lane 1 : Human fetal liver lysates
Lane 2 : Mouse liver lysates
Lane 3 : Rat liver lysates
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
All lanes : Anti-MAT1A antibody [EPR7938] (ab129176) at 1/1000 dilution (unpurified)
Lane 1 : Fetal liver lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab129176 has been referenced in 1 publication.
- Lapek JD et al. Defining Host Responses during Systemic Bacterial Infection through Construction of a Murine Organ Proteome Atlas. Cell Syst 6:579-592.e4 (2018). PubMed: 29778837