Overview

  • Product name
    Anti-MAT2A + MAT1A antibody [EPR10496]
  • Description
    Rabbit monoclonal [EPR10496] to MAT2A + MAT1A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MAT1A aa 50-150. The exact sequence is proprietary.
    Database link: P31153

  • Positive control
    • HeLa, Ramos, 293T and Human fetal liver lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Images

    • All lanes : Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484) at 1/1000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : Ramos lysate
      Lane 3 : 293T lysate
      Lane 4 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 44 kDa

    • All lanes :

      Lane 1 : Mouse spleen
      Lane 2 : Rat kidney

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 44 kDa
      Observed band size: 44 kDa


      Exposure time: 3 minutes


      Blocking and Diluting buffer and concentration: 5% NFDM/TBST

    • All lanes : Anti-MAT2A + MAT1A antibody [EPR10496] (ab177484) at 1/10000 dilution

      Lane 1 : Full-length Human MAT1A recombinant protein with His-Tag
      Lane 2 : Full-length Human MAT2A recombinant protein with GST-Tag

      Lysates/proteins at 0.015 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 44 kDa



      MAT1A: Full-length recombinant protein (aa1-395) with His-Tag was made in-house
      MAT2A: Full-length recombinant protein (aa1-364) with GST-Tag was from Abnova company

      Blocking and diluting buffer and concentrations: 5%NFDM/TBST

      Exposure time: Lane 1: 1 second
                             Lane 2: 3 seconds

       

       

    References

    ab177484 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab177484.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up