Overview

  • Product name
    Anti-MATH1/HATH1 antibody [EPR6419(2)]
    See all MATH1/HATH1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6419(2)] to MATH1/HATH1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MATH1/HATH1 aa 1-100.

  • Positive control
    • Fetal brain and HepG2 lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab168374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 38 kDa.

Target

  • Function
    Activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. May play a role in the differentiation of subsets of neural cells by activating E box-dependent transcription.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATH1 antibody
    • ATOH1 antibody
    • ATOH1_HUMAN antibody
    • Atonal homolog 1 (Drosophila) antibody
    • Atonal homolog 1 antibody
    • bHLHa14 antibody
    • Class A basic helix-loop-helix protein 14 antibody
    • hATH1 antibody
    • Helix loop helix protein hATH 1 antibody
    • Helix-loop-helix protein hATH-1 antibody
    • MATH 1 antibody
    • MATH1 antibody
    • Protein atonal homolog 1 antibody
    see all

Images

  • Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374) at 1/1000 dilution (purified) + Human fetal brain tissue lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374) at 1/2000 dilution (purified) + HepG2 cell lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374) at 1/1000 dilution (purified) + Rat brain tissue lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374) at 1/1000 dilution (purified) + Mouse brain tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374) at 1/1000 dilution (unpurified)

    Lane 1 : Fetal brain lysate
    Lane 2 : HepG2 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 38 kDa

References

This product has been referenced in:
  • Akgül S  et al. Opposing Tumor-Promoting and -Suppressive Functions of Rictor/mTORC2 Signaling in Adult Glioma and Pediatric SHH Medulloblastoma. Cell Rep 24:463-478.e5 (2018). Read more (PubMed: 29996106) »
  • Wölnerhanssen BK  et al. Deregulation of transcription factors controlling intestinal epithelial cell differentiation; a predisposing factor for reduced enteroendocrine cell number in morbidly obese individuals. Sci Rep 7:8174 (2017). Read more (PubMed: 28811552) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (colorectal cancer)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
250000 cells
Specification
colorectal cancer
Blocking step
Odyssey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C

Dr. Ron Smits

Verified customer

Submitted Jan 15 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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