Product nameAnti-MATH2/NEUROD6 antibody
See all MATH2/NEUROD6 primary antibodies
DescriptionRabbit polyclonal to MATH2/NEUROD6
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Monkey
Synthetic peptide corresponding to Human MATH2/NEUROD6 aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- Purchase matching WB positive control:Recombinant Human MATH2/NEUROD6 protein
- This antibody gave a positive signal in the following tissue lysates: Human brain; Mouse brain; Rat brain, as well as the following whole cell lysates: PC12; U87mg; U373 MG Nuclear. ICC/IF: U-87 MG cell line.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionActivates E box-dependent transcription in collaboration with TCF3/E47. May be a trans-acting factor involved in the development and maintenance of the mammalian nervous system. Transactivates the promoter of its own gene.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
- Information by UniProt
- Atoh 2 antibody
- Atoh2 antibody
- Atonal homolog 2 antibody
All lanes : Anti-MATH2/NEUROD6 antibody (ab85824) at 1 µg/ml
Lane 1 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 2 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Brain (Rat) Tissue Lysate
Lane 5 : Human brain tissue lysate - total protein (ab29466)
Lane 6 : U373 MG (Human glioblastoma-astrocytoma, epithelial-like cell line) Nuclear Lysate (ab14902)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 120 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
ab85824 staining MATH2/NEUROD6 in U-87 MG cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with the antibody ab85824 at 5µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.
This product has been referenced in:
- Komura H et al. Alzheimer Aß Assemblies Accumulate in Excitatory Neurons upon Proteasome Inhibition and Kill Nearby NAKa3 Neurons by Secretion. iScience 13:452-477 (2019). Read more (PubMed: 30827871) »
- Sullivan CS et al. Perineuronal Net Protein Neurocan Inhibits NCAM/EphA3 Repellent Signaling in GABAergic Interneurons. Sci Rep 8:6143 (2018). Read more (PubMed: 29670169) »