Overview

  • Product name

    Anti-MATH2/NEUROD6 antibody
    See all MATH2/NEUROD6 primary antibodies
  • Description

    Rabbit polyclonal to MATH2/NEUROD6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human MATH2/NEUROD6 aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab95917)

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab85824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Activates E box-dependent transcription in collaboration with TCF3/E47. May be a trans-acting factor involved in the development and maintenance of the mammalian nervous system. Transactivates the promoter of its own gene.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Atoh 2 antibody
    • Atoh2 antibody
    • Atonal homolog 2 antibody
    • Atonal protein homolog 2 antibody
    • Atonal protein homolog2 antibody
    • bHLH a2 antibody
    • bHLHa 2 antibody
    • bHLHa2 antibody
    • Class A basic helix-loop-helix protein 2 antibody
    • Helix loop helix protein mATH 2 antibody
    • Helix loop helix protein mATH2 antibody
    • MATH 2 antibody
    • Math2 antibody
    • NDF 6 antibody
    • NDF6 antibody
    • NDF6_HUMAN antibody
    • Neuro D6 antibody
    • NeuroD6 antibody
    • Neurogenic differentiation 6 antibody
    • Neurogenic differentiation factor 6 antibody
    • Nex 1 antibody
    • Nex 1m antibody
    • Nex antibody
    • Nex1 antibody
    • Nex1 m antibody
    • Nex1m antibody
    • Protein atonal homolog 2 antibody
    • Protein atonal homolog2 antibody
    see all

Images

  • All lanes : Anti-MATH2/NEUROD6 antibody (ab85824) at 1 µg/ml

    Lane 1 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 2 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Brain (Rat) Tissue Lysate
    Lane 5 : Human brain tissue lysate - total protein (ab29466)
    Lane 6 : U373 MG (Human glioblastoma-astrocytoma, epithelial-like cell line) Nuclear Lysate (ab14902)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 100 kDa, 120 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 16 minutes
  • ab85824 staining MATH2/NEUROD6 in U-87 MG cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with the antibody ab85824 at 5µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.

References

This product has been referenced in:

  • Komura H  et al. Alzheimer Aß Assemblies Accumulate in Excitatory Neurons upon Proteasome Inhibition and Kill Nearby NAKa3 Neurons by Secretion. iScience 13:452-477 (2019). Read more (PubMed: 30827871) »
  • Sullivan CS  et al. Perineuronal Net Protein Neurocan Inhibits NCAM/EphA3 Repellent Signaling in GABAergic Interneurons. Sci Rep 8:6143 (2018). Read more (PubMed: 29670169) »
See all 4 Publications for this product

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