Product nameAnti-Matrin 3 antibody
See all Matrin 3 primary antibodies
DescriptionRabbit polyclonal to Matrin 3
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Matrin 3 aa 800-847 (C terminal). The exact sequence is proprietary.
Database link: P43243
- IHC-P: Human laryngeal squamous cell carcinoma. Mouse squamous cell carcinoma tissue. WB: HeLa, HEK-293T and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate. IF: Human stomach carcinoma.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/15000.|
|IP||Use at 1-4 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionMay play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. In association with the SFPQ-NONO heteromer may play a role in nuclear retention of defective RNAs.
Involvement in diseaseDefects in MATR3 are the cause of myopathy distal type 2 (MPD2) [MIM:606070]; also called vocal cord and pharyngeal dysfunction with distal myopathy (VCPDM). MPD2 is a muscular disorder characterized by distal weakness, with onset in hands and feet, associated with vocal cord and pharyngeal weakness causing a nasal voice and swallowing disorders.
Sequence similaritiesContains 1 matrin-type zinc finger.
Contains 2 RRM (RNA recognition motif) domains.
Cellular localizationNucleus matrix.
- Information by UniProt
- ALS21 antibody
- KIAA0723 antibody
- Matr3 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human laryngeal squamous cell carcinoma (Left panel) and Mouse squamous cell carcinoma (Right panel) labeling Matrin 3 with ab264136 at 1/1,000 dilution. Detection: DAB.
All lanes : Anti-Matrin 3 antibody (ab264136) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Lane 5 : NIH/3T3 whole cell lysate at 50 µg
Exposure time: 30 seconds
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human carcinoma tissue labeling Matrin 3 (Red) with ab264136 at 1/400 dilution. Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed antibody DyLight® 594 conjugated used at a dilution of 1/100.
Matrin 3 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264136 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264136 at 1 µg/ml.
Lane 1: ab264136 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 3 secs.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264136 has not yet been referenced specifically in any publications.