Overview

  • Product name
  • Description
    Rabbit polyclonal to Matrin 3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    A region within synthetic peptide: MSKSFQQSSL SRDSQGHGRD LSAAGIGLLA AATQSLSMPA SLGRMNQGTA, corresponding to N terminal amino acids 1-50 of Human Matrin 3

  • Positive control
    • Jurkat cell lysate, Human heart tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab51081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function
    May play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. In association with the SFPQ-NONO heteromer may play a role in nuclear retention of defective RNAs.
  • Involvement in disease
    Defects in MATR3 are the cause of myopathy distal type 2 (MPD2) [MIM:606070]; also called vocal cord and pharyngeal dysfunction with distal myopathy (VCPDM). MPD2 is a muscular disorder characterized by distal weakness, with onset in hands and feet, associated with vocal cord and pharyngeal weakness causing a nasal voice and swallowing disorders.
  • Sequence similarities
    Contains 1 matrin-type zinc finger.
    Contains 2 RRM (RNA recognition motif) domains.
  • Cellular localization
    Nucleus matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALS21 antibody
    • KIAA0723 antibody
    • Matr3 antibody
    • MATR3_HUMAN antibody
    • Matrin-3 antibody
    • Matrin3 antibody
    • MPD2 antibody
    • VCPDM antibody
    • Vocal cord and pharyngeal weakness with distal myopathy antibody
    see all

Images

  • Anti-Matrin 3 antibody (ab51081) at 1.25 µg + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 95 kDa
    Observed band size: 95 kDa

  • Ab51081 at 8µg/ml staining myocardial cells of paraffin embedded human heart tissue.
  • Ab51081 at 8µg/ml staining paraffin embedded human skeletal muscle cells.

References

This product has been referenced in:
  • Rajgor D  et al. Identification of novel nesprin-1 binding partners and cytoplasmic matrin-3 in processing bodies. Mol Biol Cell 27:3894-3902 (2016). WB, ICC/IF ; Human . Read more (PubMed: 27733621) »
  • Depreux FF  et al. Disruption of the lamin A and matrin-3 interaction by myopathic LMNA mutations. Hum Mol Genet 24:4284-95 (2015). Read more (PubMed: 25948554) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for contacting us.

I am sorry to hear of the troubles which you have been experiencing using our products. Could please provide me with details of your western blotting protocol as well as tissue type and preparation steps and any troubleshooting steps which you have performed? After review of these details I may be able to offer recommendations which would improve the results which you have been experiencing. Should we not be able to improve these results, these products are covered by our Abpromise guarantee. We provide replacement or refund should this product not perform as indicated on the datasheet. More information on our Abpromise may be found at the following link:

https://www.abcam.com/index.html?pageconfig=abpromise

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you very much for letting me know.

I wish you all the best with your experiments and look forward to hearing how you get on.

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Answer

Thank you for getting back in contact.

I can understand why you would like to test both antibodies at the same time, and therefore I am willing to offer you this as a goodwill gesture. In order to do this I will need the original order number for ab70336. If you do not have this to hand, a delivery address and an approximate date of delivery should be enough. If you can get this information to me before 4 pm (Netherlands time) I will be able to set up the shipment to be with you tomorrow.

I would appreciate it though, if you do have trouble with the western blotting (particularly ab70336) if you could let me know prior to publishing the Abreview as there may be suggestions I can make in optimising the protocol.

I look forward to receiving your response.

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Answer

Thank you for contacting us and I'm sorry in the delay in getting back to you.

As discussed over the phone yesterday, I am seeking more information from the source of this antibody to ascertain exactly why ab70336 was not deemed suitable for use in Western blotting. Once I have more information in this regard I will let you know.

Ab70336 has been used to detect both the mouse and human protein. This is the only anti-Matrin 3 antibody from our catalogue which has been used to detect the mouse protein. https://www.abcam.com/Matrin-3-antibody-ab51081.html, which has been validated in western blotting is however predicted to react with the mouse protein due to the sequence homology between the immunogen used to raise the antibody and the mouseMatrin 3 protein. These sequences share 98% homology (please refer to the attachment of this email). As mouse samples have not been tested with this antibody, I cannot however guarantee that it will.

I think I would suggest it would be worth trying ab70336 with your mouse samples in western blotting. If you find that it does not work then I can offer you ab51081 free of charge to try, if you will submit an Abreview for the two antibodies.This will only take 5 minutes to submit and would be invaluable data to our other customers looking for similar Matrin 3 antibodies as yourself.

I hope this information has been of help. I look forward to hearing how you would like to proceed.

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Question
Answer

Thank you for confirming those details. I have now set up a new delivery for you to the address which you specified. This should be with you tomorrow, or at the latest Friday. The order number is xxxx (PO number FOCR xxxxx).

You should get confirmation of the Fedex tracking number once the shipment has been sent.

I look forward to hearing how you get on with your experiments. Until then I wish you all the best.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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