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We have ordered a couple of weeks ago 3 Abs for matrin3, TRBP2 and Mov10 proteins. We used those Abs for WB and none of them worked. TRBP2 (AB72110) it doesnt give any band after o/n or 2-3h RT even in 1/500 dilution and matrin3 (ab51081) Mov10 (ab80613) they really give many many bands but not the right ones. These Abs came together, is it possible that sth happen during transfer? Would you please help us with make them work? Alternatively, could you please consider change them with another lot that you know is working or another Ab for these mouse proteins? We are looking forward for your reply in your earliest convenience!! Thank you in advance!!
Asked on May 21 2012
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I am sorry to hear of the troubles which you have been experiencing using our products. Could please provide me with details of your western blotting protocol as well as tissue type and preparation steps and any troubleshooting steps which you have performed? After review of these details I may be able to offer recommendations which would improve the results which you have been experiencing. Should we not be able to improve these results, these products are covered by our Abpromise guarantee. We provide replacement or refund should this product not perform as indicated on the datasheet. More information on our Abpromise may be found at the following link:
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Answered on May 21 2012