Overview

  • Product name

  • Description

    Rabbit polyclonal to Matrin 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Mouse, Human, Xenopus laevis
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide corresponding to a region between residues 475 and 500 of human Matrin 3 (NP_061322.2)

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab70336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 1-4 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      May play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. In association with the SFPQ-NONO heteromer may play a role in nuclear retention of defective RNAs.
    • Involvement in disease

      Defects in MATR3 are the cause of myopathy distal type 2 (MPD2) [MIM:606070]; also called vocal cord and pharyngeal dysfunction with distal myopathy (VCPDM). MPD2 is a muscular disorder characterized by distal weakness, with onset in hands and feet, associated with vocal cord and pharyngeal weakness causing a nasal voice and swallowing disorders.
    • Sequence similarities

      Contains 1 matrin-type zinc finger.
      Contains 2 RRM (RNA recognition motif) domains.
    • Cellular localization

      Nucleus matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • ALS21 antibody
      • KIAA0723 antibody
      • Matr3 antibody
      • MATR3_HUMAN antibody
      • Matrin-3 antibody
      • Matrin3 antibody
      • MPD2 antibody
      • VCPDM antibody
      • Vocal cord and pharyngeal weakness with distal myopathy antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human laryengeal squamous cell carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling Matrin 3 with ab70336 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of Human Matrin 3 by Western Blot of Immunprecipitate. Lane 1: HeLa whole cell lysates immunoprecipitated using ab70336 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 2: an irrelevant antibody was used for immunoprecipitation. All lanes: ab70336 at 3µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

    References

    This product has been referenced in:

    • Boehringer A  et al. ALS Associated Mutations in Matrin 3 Alter Protein-Protein Interactions and Impede mRNA Nuclear Export. Sci Rep 7:14529 (2017). Read more (PubMed: 29109432) »
    • Li Y  et al. Immunoprecipitation and mass spectrometry defines an extensive RBM45 protein-protein interaction network. Brain Res 1647:79-93 (2016). Read more (PubMed: 26979993) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Answer

    Thank you very much for letting me know.

    I wish you all the best with your experiments and look forward to hearing how you get on.

    Read More

    Question
    Answer

    Thank you for confirming those details. I have now set up a new delivery for you to the address which you specified. This should be with you tomorrow, or at the latest Friday. The order number is xxxx (PO number FOCR xxxxx).

    You should get confirmation of the Fedex tracking number once the shipment has been sent.

    I look forward to hearing how you get on with your experiments. Until then I wish you all the best.

    Read More

    Answer

    Thank you for getting back in contact.

    I can understand why you would like to test both antibodies at the same time, and therefore I am willing to offer you this as a goodwill gesture. In order to do this I will need the original order number for ab70336. If you do not have this to hand, a delivery address and an approximate date of delivery should be enough. If you can get this information to me before 4 pm (Netherlands time) I will be able to set up the shipment to be with you tomorrow.

    I would appreciate it though, if you do have trouble with the western blotting (particularly ab70336) if you could let me know prior to publishing the Abreview as there may be suggestions I can make in optimising the protocol.

    I look forward to receiving your response.

    Read More

    Answer

    Thank you for contacting us and I'm sorry in the delay in getting back to you.

    As discussed over the phone yesterday, I am seeking more information from the source of this antibody to ascertain exactly why ab70336 was not deemed suitable for use in Western blotting. Once I have more information in this regard I will let you know.

    Ab70336 has been used to detect both the mouse and human protein. This is the only anti-Matrin 3 antibody from our catalogue which has been used to detect the mouse protein. https://www.abcam.com/Matrin-3-antibody-ab51081.html, which has been validated in western blotting is however predicted to react with the mouse protein due to the sequence homology between the immunogen used to raise the antibody and the mouseMatrin 3 protein. These sequences share 98% homology (please refer to the attachment of this email). As mouse samples have not been tested with this antibody, I cannot however guarantee that it will.

    I think I would suggest it would be worth trying ab70336 with your mouse samples in western blotting. If you find that it does not work then I can offer you ab51081 free of charge to try, if you will submit an Abreview for the two antibodies.This will only take 5 minutes to submit and would be invaluable data to our other customers looking for similar Matrin 3 antibodies as yourself.

    I hope this information has been of help. I look forward to hearing how you would like to proceed.

    Read More
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (293T)
    Total protein in input
    10 µg
    Specification
    293T
    Immuno-precipitation step
    Protein A/G

    Dr. Alessandro Marcello

    Verified customer

    Submitted Sep 30 2011

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