The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Note: expected to work on frozen sections.
May play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. In association with the SFPQ-NONO heteromer may play a role in nuclear retention of defective RNAs.
Involvement in disease
Defects in MATR3 are the cause of myopathy distal type 2 (MPD2) [MIM:606070]; also called vocal cord and pharyngeal dysfunction with distal myopathy (VCPDM). MPD2 is a muscular disorder characterized by distal weakness, with onset in hands and feet, associated with vocal cord and pharyngeal weakness causing a nasal voice and swallowing disorders.
Detection of Human Matrin 3 by Immunofluorescence. Sample: FFPE section of human breast carcinoma.Antibody: ab84422 used at a dilution of 1/100. Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG used at a dilution of 1/500.
Detection of Human Matrin 3 by Immunofluorescence. Sample: FFPE section of Human breast carcinoma.Antibody: ab84422 used at a dilution of 1/100. Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG used at a dilution of 1/500.
Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody (ab84422)Image courtesy of Leilani Beltran by Abreview.
ab84422 staining Matrin 3 in human U2OS cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde, permeabilized using 0.5% NP-40, blocked with 1% BSA for 1 hour a room temperature, then incubated with ab84422 at a 1/100 dilution for 1 hour. The secondary antibody used was an Alexa-Fluor 488 conjugate donkey polyclonal, used at a 1/1000 dilution.