Overview

  • Product name

    Anti-Matriptase 2 antibody - Cytoplasmic domain
    See all Matriptase 2 primary antibodies
  • Description

    Rabbit polyclonal to Matriptase 2 - Cytoplasmic domain
  • Host species

    Rabbit
  • Specificity

    ab56180 reacts with Matripase 2 (TMPRSS6) and does not react with the other TMPRSSs. ab56180 recognizes all of the listed forms of Matripase 2.
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide based on the aminoterminal end (cytoplasmic domain) of Matripase 2 (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab56180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/1000 - 1/5000. Predicted molecular weight: 89 kDa.

Target

  • Function

    Serine protease which hydrolyzes a range of proteins including type I collagen, fibronectin and fibrinogen. Can also activate urokinase-type plasminogen activator with low efficiency. May play a specialized role in matrix remodeling processes in liver. Required to sense iron deficiency. Overexpression suppresses activation of the HAMP promoter.
  • Tissue specificity

    Liver specific.
  • Involvement in disease

    Defects in TMPRSS6 are the cause of iron-refractory iron deficiency anemia (IRIDA) [MIM:206200]; also known as hypochromic microcytic anemia with defect in iron metabolism or hereditary iron-handling disorder or pseudo-iron-deficiency anemia. Key features include congenital hypochromic microcytic anemia, very low mean corpuscular erythrocyte volume, low transferrin saturation, abnormal iron absorption characterized by no hematologic improvement following treatment with oral iron, and abnormal iron utilization characterized by a sluggish, incomplete response to parenteral iron.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 2 CUB domains.
    Contains 3 LDL-receptor class A domains.
    Contains 1 peptidase S1 domain.
  • Domain

    Cytoplasmic domain mediates HAMP suppression via proximal promoter element(s).
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • IRIDA antibody
    • Matriptase-2 antibody
    • Membrane bound mosaic serine proteinase matriptase 2 antibody
    • Membrane type serine proteinase 2 antibody
    • MTSP 2 antibody
    • MTSP2 antibody
    • PVAE354 antibody
    • TMPRSS 6 antibody
    • TMPRSS6 antibody
    • TMPS6_HUMAN antibody
    • TMSP 6 antibody
    • TMSP6 antibody
    • Transmembrane protease serine 6 antibody
    • Type II Membrane Serine Proteinase 6 antibody
    see all

Images

  • All lanes : Anti-Matriptase 2 antibody - Cytoplasmic domain (ab56180) at 1/1000 dilution

    All lanes : Cell media from human embryonic kidney HEK293 cells

    Predicted band size: 89 kDa

References

This product has been referenced in:

  • Lamothe SM  et al. The Human Ether-a-go-go-related Gene (hERG) Potassium Channel Represents an Unusual Target for Protease-mediated Damage. J Biol Chem 291:20387-401 (2016). Read more (PubMed: 27502273) »
  • Foka P  et al. A complex signaling network involving protein kinase CK2 is required for hepatitis C virus core protein-mediated modulation of the iron-regulatory hepcidin gene expression. Cell Mol Life Sci N/A:N/A (2014). WB . Read more (PubMed: 24718935) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Cell lysate - whole cell (Hepatoma cells)
Specification
Hepatoma cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C

Dr. Alexios Dimitriadis

Verified customer

Submitted Jul 15 2014

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T transfected with Hu matriptase 2.FLAG)
Total protein in input
1e+007 cells
Specification
293T transfected with Hu matriptase 2.FLAG
Immuno-precipitation step
Protein A

Ms. Schimanski Lisa

Verified customer

Submitted Oct 22 2008

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