Product nameAnti-Matrix protein 1 antibody
See all Matrix protein 1 primary antibodies
DescriptionGoat polyclonal to Matrix protein 1
Tested applicationsSuitable for: ICC/IF, ELISA, Conjugation, WBmore details
Unsuitable for: IHC
Species reactivityReacts with: Species independent
Full length native protein (purified) corresponding to Matrix protein 1. Purified M1 protein, Influenza A-Phillipines (H3N2).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityIon Exchange Chromatography
Purification notes>95% pure. Sodium sulfate precipitation and ion-exchange chromatography.
Our Abpromise guarantee covers the use of ab20910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|ELISA||Use at an assay dependent dilution.|
|Conjugation||Use at an assay dependent dilution.|
|WB||1/500. Detects a band of approximately 27,54 kDa (predicted molecular weight: 27,54 kDa).|
RelevanceInfluenza virus type A matrix protein, also known as M1, is composed of a 252 amino acid sequence and is type-specific in influenza viruses. It is located inside the viral lipid envelope and plays a key role in virus assembly and replication. M1 can be isolated from particles by removing the envelope with detergents and reducing the pH to 4.0. Influenza viruses are a common and widely spread infectious agent. Like many other viruses, influenza virus are constantly undergoing mutations and thereby avoiding the immune system. The Influenza A Virus M proteins form a continuous shell on the inner side of the lipid bilayer, maintaining the structural integrity of the virus particle through hydrophobic interactions.
- M1 antibody
- Matrix Protein 1 antibody
- Matrix Protein I antibody
- Membrane matrix protein M1 antibody
Immunocytochemical immunoflurescence analysis of Formaldehyde-fixed human kidney epithelial cells, labelling Influenza A Virus M1 matrix protein with ab20910 at a dilution of 1/500 incubated fro 1 hour at 18°C in 1% FCS PBS. Blocking was with 1% serum incubated for 30 minutes at 18°C. Secondary was a donkey anti-goat Alexa Fluor® 568 undiluted. Cells were transfected with either GFP-M1 or pCDNA2-NP, as indicated on the left hand side of the figure. 24h later cells were fixed and stained for M1 using ab20910 (red) or NP using ab20343 (grey). The abcam 20910 detected M1 in cells transfected with GFP-M1 but not pCDNA3-NP. Moreover, the level of co-localization between GFP-M1 and ab20910 was quite good.
All lanes : Anti-Matrix protein 1 antibody (ab20910) at 1/500 dilution
Lane 1 : 293T cells transfected with GFP-M1 and infected with influenza A virus
Lane 2 : 293T cells transfected with GFP-M1 and mock infected
Lane 3 : 293T cells transfected with pcDNA-NP and infected with influenza A virus
Lane 4 : 293T cells transfected with pcDNA-NP and mock infected
Lane 5 : 293T cells infected with influenza A virus
Lane 6 : 293T cells mock infected
Lane 7 : M1 expressed using a minireplicon system
Lane 8 : Minireplicon system control (no PB2)
All lanes : Donkey anti-goat (IRDye® 800CW) at 1/10000 dilution
Predicted band size: 27,54 kDa
Observed band size: 27,54 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Alenquer M et al. Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites. Nat Commun 10:1629 (2019). Read more (PubMed: 30967547) »
- Sequeira DP et al. Combining stable insect cell lines with baculovirus-mediated expression for multi-HA influenza VLP production. Vaccine 36:3112-3123 (2018). WB . Read more (PubMed: 28291648) »