Key features and details
- Goat polyclonal to Matrix protein 1
- Suitable for: ICC/IF, ELISA
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-Matrix protein 1 antibody
See all Matrix protein 1 primary antibodies
DescriptionGoat polyclonal to Matrix protein 1
Tested applicationsSuitable for: ICC/IF, ELISAmore details
Unsuitable for: IHC
Species reactivityReacts with: Species independent
Full length native protein (purified) corresponding to Matrix protein 1. Purified M1 protein, Influenza A-Phillipines (H3N2).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityIon Exchange Chromatography
Purification notes>95% pure. Sodium sulfate precipitation and ion-exchange chromatography.
Our Abpromise guarantee covers the use of ab20910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
RelevanceInfluenza virus type A matrix protein, also known as M1, is composed of a 252 amino acid sequence and is type-specific in influenza viruses. It is located inside the viral lipid envelope and plays a key role in virus assembly and replication. M1 can be isolated from particles by removing the envelope with detergents and reducing the pH to 4.0. Influenza viruses are a common and widely spread infectious agent. Like many other viruses, influenza virus are constantly undergoing mutations and thereby avoiding the immune system. The Influenza A Virus M proteins form a continuous shell on the inner side of the lipid bilayer, maintaining the structural integrity of the virus particle through hydrophobic interactions.
- M1 antibody
- Matrix Protein 1 antibody
- Matrix Protein I antibody
- Membrane matrix protein M1 antibody
Immunocytochemical immunoflurescence analysis of Formaldehyde-fixed human kidney epithelial cells, labelling Influenza A Virus M1 matrix protein with ab20910 at a dilution of 1/500 incubated fro 1 hour at 18°C in 1% FCS PBS. Blocking was with 1% serum incubated for 30 minutes at 18°C. Secondary was a donkey anti-goat Alexa Fluor® 568 undiluted. Cells were transfected with either GFP-M1 or pCDNA2-NP, as indicated on the left hand side of the figure. 24h later cells were fixed and stained for M1 using ab20910 (red) or NP using ab20343 (grey). The abcam 20910 detected M1 in cells transfected with GFP-M1 but not pCDNA3-NP. Moreover, the level of co-localization between GFP-M1 and ab20910 was quite good.
ab20910 has been referenced in 9 publications.
- Alenquer M et al. Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites. Nat Commun 10:1629 (2019). PubMed: 30967547
- Tome-Amat J et al. Influenza A Virus Utilizes Low-Affinity, High-Avidity Interactions with the Nuclear Import Machinery To Ensure Infection and Immune Evasion. J Virol 93:N/A (2019). PubMed: 30305352
- Sequeira DP et al. Combining stable insect cell lines with baculovirus-mediated expression for multi-HA influenza VLP production. Vaccine 36:3112-3123 (2018). WB . PubMed: 28291648
- Slater T et al. Bat lung epithelial cells show greater host species-specific innate resistance than MDCK cells to human and avian influenza viruses. Virol J 15:68 (2018). WB . PubMed: 29636078
- Carvalho SB et al. Bioorthogonal Strategy for Bioprocessing of Specific-Site-Functionalized Enveloped Influenza-Virus-Like Particles. Bioconjug Chem N/A:N/A (2016). WB ; Influenza A . PubMed: 27652605
- Vale-Costa S et al. Influenza A virus ribonucleoproteins modulate host recycling by competing with Rab11 effectors. J Cell Sci 129:1697-710 (2016). PubMed: 26940915
- Kim D et al. Correlative stochastic optical reconstruction microscopy and electron microscopy. PLoS One 10:e0124581 (2015). ICC ; Goat . PubMed: 25874453
- He J et al. Dual function of CD81 in influenza virus uncoating and budding. PLoS Pathog 9:e1003701 (2013). PubMed: 24130495
- Das SC et al. The highly conserved arginine residues at positions 76 through 78 of influenza A virus matrix protein M1 play an important role in viral replication by affecting the intracellular localization of M1. J Virol 86:1522-30 (2012). ICC/IF ; Influenza A . PubMed: 22090133