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    matrix-protein-1-antibody-ga2b-ab22396.pdf

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Microbiology Organism Virus RNA Virus single stranded RNA negative strand virus Influenza
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Anti-Matrix protein 1 antibody [GA2B] (ab22396)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (19)

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Immunocytochemistry/ Immunofluorescence - Anti-Matrix protein 1 antibody [GA2B] (ab22396)

    Key features and details

    • Mouse monoclonal [GA2B] to Matrix protein 1
    • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
    • Reacts with: Species independent
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-Matrix protein 1 antibody [GA2B]
      See all Matrix protein 1 primary antibodies
    • Description

      Mouse monoclonal [GA2B] to Matrix protein 1
    • Host species

      Mouse
    • Tested applications

      Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details
    • Species reactivity

      Reacts with: Species independent
    • Immunogen

      Tissue, cells or virus corresponding to Matrix protein 1. Influenza A/ Puerto Rico/ 8/ 34 (H1N1) and A/Bangkok/ 1/ 79 (H3N2) viruses

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.50
      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      SDS-PAGE
    • Purification notes

      >90% IgG content as established by SDS PAGE
    • Clonality

      Monoclonal
    • Clone number

      GA2B
    • Myeloma

      P3x63-Ag8.653
    • Isotype

      IgG1
    • Research areas

      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • single stranded RNA negative strand virus
      • Influenza
      • Microbiology
      • Protein
      • Viral Protein

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Matrix protein 1 (His tag) (ab217650)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab22396 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt (1)
    Use at an assay dependent concentration. PubMed: 20413723

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    IHC-P
    Use at an assay dependent concentration.
    WB (1)
    Use at an assay dependent concentration.
    ICC/IF
    1/100.
    Notes
    Flow Cyt
    Use at an assay dependent concentration. PubMed: 20413723

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    IHC-P
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration.
    ICC/IF
    1/100.

    Target

    • Relevance

      Influenza virus type A matrix protein, also known as M1, is composed of a 252 amino acid sequence and is type-specific in influenza viruses. It is located inside the viral lipid envelope and plays a key role in virus assembly and replication. M1 can be isolated from particles by removing the envelope with detergents and reducing the pH to 4.0. Influenza viruses are a common and widely spread infectious agent. Like many other viruses, influenza virus are constantly undergoing mutations and thereby avoiding the immune system. The Influenza A Virus M proteins form a continuous shell on the inner side of the lipid bilayer, maintaining the structural integrity of the virus particle through hydrophobic interactions.
    • Cellular localization

      Cytoplasmic
    • Alternative names

      • M1 antibody
      • Matrix Protein 1 antibody
      • Matrix Protein I antibody
      • Membrane matrix protein M1 antibody

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Matrix protein 1 antibody [GA2B] (ab22396)
      Immunocytochemistry/ Immunofluorescence - Anti-Matrix protein 1 antibody [GA2B] (ab22396)Image from Chase GP et al., PLoS Pathog. 2011 Sep;7(9):e1002187. Epub 2011 Sep 1.Fig 4.; doi:10.1371/journal.ppat.1002187; September 1, 2011, PLoS Pathog 7(9): e1002187.
      Immunofluorescence analysis of HeLa cells staining Influenza A Virus M1 using ab22396.

      Cells were treated with either 20 µg/ml Prostaglandin A (PGA) or EtOH vehicle control, 3 hours post infection by Influenza A Virus, then fractionated at 9 hours post infection before analysis by immunofluorescence.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (19)

    Publishing research using ab22396? Please let us know so that we can cite the reference in this datasheet.

    ab22396 has been referenced in 19 publications.

    • Amarelle L  et al. Cardiac glycosides decrease influenza virus replication by inhibiting cell protein translational machinery. Am J Physiol Lung Cell Mol Physiol 316:L1094-L1106 (2019). PubMed: 30892074
    • Xie X  et al. Influenza Vaccine With Consensus Internal Antigens as Immunogens Provides Cross-Group Protection Against Influenza A Viruses. Front Microbiol 10:1630 (2019). PubMed: 31379782
    • Kang HJ  et al. Influenza M2 virus-like particle vaccination enhances protection in combination with avian influenza HA VLPs. PLoS One 14:e0216871 (2019). PubMed: 31246961
    • Vashisht S  et al. Cross-clade antibody reactivity may attenuate the ability of influenza virus to evade the immune response. Mol Immunol 114:149-161 (2019). PubMed: 31352231
    • Desforges JP  et al. Environmental contaminant mixtures modulate in vitro influenza infection. Sci Total Environ 634:20-28 (2018). Flow Cyt . PubMed: 29626767
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-Matrix protein 1 antibody [GA2B]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Influenza A Tissue lysate - other (X-31 influenza virus grown in eggs, sucrose purifi)
    Gel Running Conditions
    Reduced Denaturing (4-12% gel)
    Loading amount
    4 µg
    Specification
    X-31 influenza virus grown in eggs, sucrose purifi
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 09 2020

    Flow Cytometry abreview for Anti-Influenza A Virus M1 antibody [GA2B]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Influenza infected A549)
    Specification
    Influenza infected A549
    Preparation
    Cell harvesting/tissue preparation method: Trypsinised
    Sample buffer: PBS + 1%BSA + EDTA
    Fixation
    Paraformaldehyde
    Permeabilization
    Yes - BD Fix/Perm Kit
    Gating Strategy
    Single cell suspension
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 06 2011

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