Anti-Matrix protein 1 antibody [GA2B] (ab22396)

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Key features and details

  • Mouse monoclonal [GA2B] to Matrix protein 1
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
  • Reacts with: Species independent
  • Isotype: IgG1

Overview

  • Product name

    Anti-Matrix protein 1 antibody [GA2B]
    See all Matrix protein 1 primary antibodies

  • Description

    Mouse monoclonal [GA2B] to Matrix protein 1

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Tissue/ cell preparation- Influenza A/ Puerto Rico/ 8/ 34 (H1N1) and A/Bangkok/ 1/ 79 (H3N2) viruses

Properties

Applications

Our Abpromise guarantee covers the use of ab22396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 20413723

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ICC/IF 1/100.

Target

  • Relevance

    Influenza virus type A matrix protein, also known as M1, is composed of a 252 amino acid sequence and is type-specific in influenza viruses. It is located inside the viral lipid envelope and plays a key role in virus assembly and replication. M1 can be isolated from particles by removing the envelope with detergents and reducing the pH to 4.0. Influenza viruses are a common and widely spread infectious agent. Like many other viruses, influenza virus are constantly undergoing mutations and thereby avoiding the immune system. The Influenza A Virus M proteins form a continuous shell on the inner side of the lipid bilayer, maintaining the structural integrity of the virus particle through hydrophobic interactions.

  • Cellular localization

    Cytoplasmic

  • Alternative names

    • M1 antibody
    • Matrix Protein 1 antibody
    • Matrix Protein I antibody
    • Membrane matrix protein M1 antibody

Images

  • Flow Cytometry - Anti-Matrix protein 1 antibody [GA2B] (ab22396)
    Flow Cytometry - Anti-Matrix protein 1 antibody [GA2B] (ab22396)Image courtesy of an anonymous Abreview.
    ab22396 used in Flow Cytometry.
    Influenza infected A549 cells were trypsinized, fixed in paraformaldehyde, permeabilized and then incubated with ab22396 for analysis by Flow Cytometry.

    ab22396 used at a 1/200 dilution.
    The secondary used was ab96874, diluted 1/200.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Matrix protein 1 antibody [GA2B] (ab22396)
    Immunocytochemistry/ Immunofluorescence - Anti-Matrix protein 1 antibody [GA2B] (ab22396)Image from Chase GP et al., PLoS Pathog. 2011 Sep;7(9):e1002187. Epub 2011 Sep 1.Fig 4.; doi:10.1371/journal.ppat.1002187; September 1, 2011, PLoS Pathog 7(9): e1002187.
    Immunofluorescence analysis of HeLa cells staining Influenza A Virus M1 using ab22396.

    Cells were treated with either 20 µg/ml Prostaglandin A (PGA) or EtOH vehicle control, 3 hours post infection by Influenza A Virus, then fractionated at 9 hours post infection before analysis by immunofluorescence.

References

Publishing research using ab22396? Please let us know so that we can cite the reference in this datasheet.

ab22396 has been referenced in 16 publications.

  • Amarelle L  et al. Cardiac glycosides decrease influenza virus replication by inhibiting cell protein translational machinery. Am J Physiol Lung Cell Mol Physiol 316:L1094-L1106 (2019). PubMed: 30892074
  • Xie X  et al. Influenza Vaccine With Consensus Internal Antigens as Immunogens Provides Cross-Group Protection Against Influenza A Viruses. Front Microbiol 10:1630 (2019). PubMed: 31379782
  • Kang HJ  et al. Influenza M2 virus-like particle vaccination enhances protection in combination with avian influenza HA VLPs. PLoS One 14:e0216871 (2019). PubMed: 31246961
  • Desforges JP  et al. Environmental contaminant mixtures modulate in vitro influenza infection. Sci Total Environ 634:20-28 (2018). Flow Cyt . PubMed: 29626767
  • Nogales A  et al. Rearrangement of Influenza Virus Spliced Segments for the Development of Live-Attenuated Vaccines. J Virol 90:6291-302 (2016). WB . PubMed: 27122587
View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Matrix protein 1 antibody [GA2B]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Influenza A Tissue lysate - other (X-31 influenza virus grown in eggs, sucrose purifi)
Gel Running Conditions
Reduced Denaturing (4-12% gel)
Loading amount
4 µg
Specification
X-31 influenza virus grown in eggs, sucrose purifi
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
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Abcam user community

Verified customer

Submitted Jun 09 2020

Flow Cytometry abreview for Anti-Influenza A Virus M1 antibody [GA2B]

 Good
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (Influenza infected A549)
Specification
Influenza infected A549
Preparation
Cell harvesting/tissue preparation method: Trypsinised
Sample buffer: PBS + 1%BSA + EDTA
Fixation
Paraformaldehyde
Permeabilization
Yes - BD Fix/Perm Kit
Gating Strategy
Single cell suspension
Read More

Abcam user community

Verified customer

Submitted Oct 06 2011

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