Anti-Matrix protein 1 antibody [GA2B] (ab22396)

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Immunocytochemistry/ Immunofluorescence - Anti-Matrix protein 1 antibody [GA2B] (ab22396)

    Key features and details

    • Mouse monoclonal [GA2B] to Matrix protein 1
    • Suitable for: IHC-P, WB, ICC/IF
    • Reacts with: Species independent
    • Isotype: IgG1

    Overview

    • Product name

      Anti-Matrix protein 1 antibody [GA2B]
      See all Matrix protein 1 primary antibodies

    • Description

      Mouse monoclonal [GA2B] to Matrix protein 1

    • Host species

      Mouse

    • Tested applications

      Suitable for: IHC-P, WB, ICC/IFmore details

    • Species reactivity

      Reacts with: Species independent

    • Immunogen

      Tissue/ cell preparation- Influenza A/ Puerto Rico/ 8/ 34 (H1N1) and A/Bangkok/ 1/ 79 (H3N2) viruses

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab22396 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use at an assay dependent concentration.
    WB (1)
    Use at an assay dependent concentration.
    ICC/IF
    1/100.
    Notes
    IHC-P
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration.
    ICC/IF
    1/100.

    Target

    • Relevance

      Influenza virus type A matrix protein, also known as M1, is composed of a 252 amino acid sequence and is type-specific in influenza viruses. It is located inside the viral lipid envelope and plays a key role in virus assembly and replication. M1 can be isolated from particles by removing the envelope with detergents and reducing the pH to 4.0. Influenza viruses are a common and widely spread infectious agent. Like many other viruses, influenza virus are constantly undergoing mutations and thereby avoiding the immune system. The Influenza A Virus M proteins form a continuous shell on the inner side of the lipid bilayer, maintaining the structural integrity of the virus particle through hydrophobic interactions.

    • Cellular localization

      Cytoplasmic

    • Alternative names

      • M1 antibody
      • Matrix Protein 1 antibody
      • Matrix Protein I antibody
      • Membrane matrix protein M1 antibody

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Matrix protein 1 antibody [GA2B] (ab22396)
      Immunocytochemistry/ Immunofluorescence - Anti-Matrix protein 1 antibody [GA2B] (ab22396)Image from Chase GP et al., PLoS Pathog. 2011 Sep;7(9):e1002187. Epub 2011 Sep 1.Fig 4.; doi:10.1371/journal.ppat.1002187; September 1, 2011, PLoS Pathog 7(9): e1002187.
      Immunofluorescence analysis of HeLa cells staining Influenza A Virus M1 using ab22396.

      Cells were treated with either 20 µg/ml Prostaglandin A (PGA) or EtOH vehicle control, 3 hours post infection by Influenza A Virus, then fractionated at 9 hours post infection before analysis by immunofluorescence.

    References (18)

    Publishing research using ab22396? Please let us know so that we can cite the reference in this datasheet.

    ab22396 has been referenced in 18 publications.

    • Amarelle L  et al. Cardiac glycosides decrease influenza virus replication by inhibiting cell protein translational machinery. Am J Physiol Lung Cell Mol Physiol 316:L1094-L1106 (2019). PubMed: 30892074
    • Xie X  et al. Influenza Vaccine With Consensus Internal Antigens as Immunogens Provides Cross-Group Protection Against Influenza A Viruses. Front Microbiol 10:1630 (2019). PubMed: 31379782
    • Kang HJ  et al. Influenza M2 virus-like particle vaccination enhances protection in combination with avian influenza HA VLPs. PLoS One 14:e0216871 (2019). PubMed: 31246961
    • Vashisht S  et al. Cross-clade antibody reactivity may attenuate the ability of influenza virus to evade the immune response. Mol Immunol 114:149-161 (2019). PubMed: 31352231
    • Desforges JP  et al. Environmental contaminant mixtures modulate in vitro influenza infection. Sci Total Environ 634:20-28 (2018). Flow Cyt . PubMed: 29626767
    View all Publications for this product

    Customer reviews and Q&As

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    Western blot abreview for Anti-Matrix protein 1 antibody [GA2B]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Influenza A Tissue lysate - other (X-31 influenza virus grown in eggs, sucrose purifi)
    Gel Running Conditions
    Reduced Denaturing (4-12% gel)
    Loading amount
    4 µg
    Specification
    X-31 influenza virus grown in eggs, sucrose purifi
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
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    Verified customer

    Submitted Jun 09 2020

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