Overview

  • Product name

  • Description

    Rabbit polyclonal to MAVS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MAVS (internal sequence). (NP_065797.2).
    Database link: Q7Z434

  • Positive control

    • Human brain, cortex tissue; Mouse brain cells; Rat brain tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab189109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 57 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function

    Required for innate immune defense against viruses. Acts downstream of DDX58 and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis.
  • Tissue specificity

    Present in T-cells, monocytes, epithelial cells and hepatocytes (at protein level). Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes.
  • Sequence similarities

    Contains 1 CARD domain.
  • Domain

    Both CARD and transmembrane domains are essential for antiviral function. The CARD domain is responsible for interaction with DDX58 and IFIH1.
  • Post-translational
    modifications

    Ubiquitinated; undergoes 'Lys-48'-linked polyubiquitination catalyzed by ITCH; ITCH-dependent polyubiquitination is mediated by the interaction with PCBP2 and leads to MAVS proteasomal degradation.
  • Cellular localization

    Mitochondrion outer membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CARD adapter inducing interferon beta antibody
    • CARD adaptor inducing IFN beta antibody
    • Cardif antibody
    • DKFZp666M015 antibody
    • FLJ27482 antibody
    • FLJ41962 antibody
    • IFN B promoter stimulator 1 antibody
    • Interferon beta promoter stimulator protein 1 antibody
    • Ips 1 antibody
    • IPS-1 antibody
    • Ips1 antibody
    • KIAA1271 antibody
    • MAVS antibody
    • MAVS_HUMAN antibody
    • Mitochondrial anti viral signaling protein antibody
    • Mitochondrial Antiviral Signaling antibody
    • Mitochondrial antiviral signaling protein antibody
    • Mitochondrial antiviral-signaling protein antibody
    • Putative NF kappa B activating protein 031N antibody
    • Putative NF-kappa-B-activating protein 031N antibody
    • Virus induced signaling adapter antibody
    • virus induced signaling adaptor antibody
    • Virus-induced-signaling adapter antibody
    • VISA antibody
    see all

Images

  • Lane 1 : Anti-MAVS antibody (ab189109) at 0.5 µg/ml
    Lane 2 : Anti-MAVS antibody (ab189109) at 1 µg/ml
    Lane 3 : Anti-MAVS antibody (ab189109) at 2 µg/ml

    All lanes : Rat brain tissue lysate

    Predicted band size: 57 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain, cortex tissue labelling MAVS with ab189109 at 5 µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

  • Immunofluorescent analysis of Mouse brain cells labelling MAVS with ab189109 at 20 µg/ml.

References

ab189109 has not yet been referenced specifically in any publications.

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