Product nameAnti-MAVS antibody
See all MAVS primary antibodies
DescriptionRabbit polyclonal to MAVS
Tested applicationsSuitable for: IP, IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human MAVS aa 150-200. The exact sequence is proprietary.
Database link: Q7Z434
- IHC-P: Human lung carcinoma tissue. WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/1000 - 1/5000.|
|WB||1/2000 - 1/10000.|
FunctionRequired for innate immune defense against viruses. Acts downstream of DDX58 and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis.
Tissue specificityPresent in T-cells, monocytes, epithelial cells and hepatocytes (at protein level). Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes.
Sequence similaritiesContains 1 CARD domain.
DomainBoth CARD and transmembrane domains are essential for antiviral function. The CARD domain is responsible for interaction with DDX58 and IFIH1.
modificationsUbiquitinated; undergoes 'Lys-48'-linked polyubiquitination catalyzed by ITCH; ITCH-dependent polyubiquitination is mediated by the interaction with PCBP2 and leads to MAVS proteasomal degradation.
Cellular localizationMitochondrion outer membrane.
- Information by UniProt
- CARD adapter inducing interferon beta antibody
- CARD adaptor inducing IFN beta antibody
- Cardif antibody
All lanes : Anti-MAVS antibody (ab264147) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Exposure time: 3 minutes
MAVS was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264147 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264147 at 1 µg/ml.
Lane 1: ab264147 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 30 secs.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling MAVS with ab264147 at 1/200 dilution. Detection: DAB.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264147 has not yet been referenced specifically in any publications.