Key features and details
- Rabbit polyclonal to MAVS
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MAVS antibody
See all MAVS primary antibodies
DescriptionRabbit polyclonal to MAVS
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human MAVS aa 100-200.
(Peptide available as
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
ChIP Related Products
Our Abpromise guarantee covers the use of ab31334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 57, 75 kDa (predicted molecular weight: 57 kDa).|
FunctionRequired for innate immune defense against viruses. Acts downstream of DDX58 and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis.
Tissue specificityPresent in T-cells, monocytes, epithelial cells and hepatocytes (at protein level). Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes.
Sequence similaritiesContains 1 CARD domain.
DomainBoth CARD and transmembrane domains are essential for antiviral function. The CARD domain is responsible for interaction with DDX58 and IFIH1.
modificationsUbiquitinated; undergoes 'Lys-48'-linked polyubiquitination catalyzed by ITCH; ITCH-dependent polyubiquitination is mediated by the interaction with PCBP2 and leads to MAVS proteasomal degradation.
Cellular localizationMitochondrion outer membrane.
- Information by UniProt
- CARD adapter inducing interferon beta antibody
- CARD adaptor inducing IFN beta antibody
- Cardif antibody
All lanes : Anti-MAVS antibody (ab31334) at 1 µg/ml
Lane 1 : Hela Whole Cell Lysate
Lane 2 :
Jurkat whole cell lysate (ab7899)
Lane 3 :
A431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Predicted band size: 57 kDa
Observed band size: 57,75 kDa why is the actual band size different from the predicted?
Additional bands at: 51 kDa (possible non-specific binding)
The bands at 75 and 57 kDa correspond to the bands seen in a paper by Seth et al., 2005 (See Supplementary Information). PubMed: 16125763. The band at 51 kDa may represent non-specific antibody binding.
ab31334 has been referenced in 12 publications.
- Hyun J et al. HIV and HCV augments inflammatory responses through increased TREM-1 expression and signaling in Kupffer and Myeloid cells. PLoS Pathog 15:e1007883 (2019). PubMed: 31260499
- MacDuff DA et al. HOIL1 Is Essential for the Induction of Type I and III Interferons by MDA5 and Regulates Persistent Murine Norovirus Infection. J Virol 92:N/A (2018). PubMed: 30209176
- Zhang M et al. Low level expression of the Mitochondrial Antiviral Signaling protein (MAVS) associated with long-term nonprogression in SIV-infected rhesus macaques. Virol J 15:159 (2018). PubMed: 30326919
- Loetsch C et al. Cytosolic Recognition of RNA Drives the Immune Response to Heterologous Erythrocytes. Cell Rep 21:1624-1638 (2017). WB ; Mouse . PubMed: 29117566
- Cuellar TL et al. Silencing of retrotransposons by SETDB1 inhibits the interferon response in acute myeloid leukemia. J Cell Biol 216:3535-3549 (2017). PubMed: 28887438