Product nameAnti-MAVS antibody - ChIP Grade
See all MAVS primary antibodies
DescriptionRabbit polyclonal to MAVS - ChIP Grade
Tested applicationsSuitable for: IHC-P, ChIP, ICC/IF, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human MAVS aa 100-200.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: Jurkat. A431. This antibody gave a positive signal in the following Formaldehyde fixed cell line: HEK 293.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
ChIP Related Products
Our Abpromise guarantee covers the use of ab31334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml.|
|ChIP||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 57, 75 kDa (predicted molecular weight: 57 kDa).|
|IP||Use at an assay dependent concentration. PubMed: 21068253|
FunctionRequired for innate immune defense against viruses. Acts downstream of DDX58 and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis.
Tissue specificityPresent in T-cells, monocytes, epithelial cells and hepatocytes (at protein level). Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes.
Sequence similaritiesContains 1 CARD domain.
DomainBoth CARD and transmembrane domains are essential for antiviral function. The CARD domain is responsible for interaction with DDX58 and IFIH1.
modificationsUbiquitinated; undergoes 'Lys-48'-linked polyubiquitination catalyzed by ITCH; ITCH-dependent polyubiquitination is mediated by the interaction with PCBP2 and leads to MAVS proteasomal degradation.
Cellular localizationMitochondrion outer membrane.
- Information by UniProt
- CARD adapter inducing interferon beta antibody
- CARD adaptor inducing IFN beta antibody
- Cardif antibody
All lanes : Anti-MAVS antibody - ChIP Grade (ab31334) at 1 µg/ml
Lane 1 : Hela Whole Cell Lysate
Lane 2 :
Jurkat whole cell lysate (ab7899)
Lane 3 :
A431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Predicted band size: 57 kDa
Observed band size: 57,75 kDa why is the actual band size different from the predicted?
Additional bands at: 51 kDa (possible non-specific binding)
The bands at 75 and 57 kDa correspond to the bands seen in a paper by Seth et al., 2005 (See Supplementary Information). PubMed: 16125763. The band at 51 kDa may represent non-specific antibody binding.
ICC/IF image of ab31334 stained human HEK 293 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31334, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
ab31334 staining MAVS in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% NGS, 5% BSA in PBS for 2 hours at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in diluent) for 16 hours at 4°C. A Goat anti-rabbit HRP polyclonal (1/250) was used as the secondary antibody.
MAVS was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal MAVS and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31334.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 75kDa: MAVS
This product has been referenced in:
- Hyun J et al. HIV and HCV augments inflammatory responses through increased TREM-1 expression and signaling in Kupffer and Myeloid cells. PLoS Pathog 15:e1007883 (2019). Read more (PubMed: 31260499) »
- MacDuff DA et al. HOIL1 Is Essential for the Induction of Type I and III Interferons by MDA5 and Regulates Persistent Murine Norovirus Infection. J Virol 92:N/A (2018). Read more (PubMed: 30209176) »