Anti-MAX antibody [73C5a] (ab53570)
![Western blot - Anti-MAX antibody [73C5a] (ab53570)](https://www.abcam.com/ps/products/53/ab53570/Images/ab53570-66444-anti-max-antibody-73c5a-western-blot.gif "Western blot - Anti-MAX antibody [73C5a] (ab53570)")
Key features and details
- Mouse monoclonal [73C5a] to MAX
- Suitable for: Flow Cyt, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-MAX antibody [73C5a]
See all MAX primary antibodies -
Description
Mouse monoclonal [73C5a] to MAX -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Recombinant fragment
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Immunogen
Recombinant human MAX
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab53570 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
Clonality
Monoclonal -
Clone number
73C5a -
Isotype
IgG2b -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| WB |
Recombinant fragment
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|
|
| WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 18 kDa).
|
| Notes |
|---|
|
Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 18 kDa). |
Target
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Relevance
MAX is a basic helix-loop-helix, leucine zipper protein that forms heterodimers with MYC and MAD. It is involved in regulating gene expression. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 4149 Human
- Omim: 154950 Human
- SwissProt: P61244 Human
- Unigene: 285354 Human
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Alternative names
- bHLHd4 antibody
- bHLHd5 antibody
- bHLHd6 antibody
see all
Images
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Western blot - Anti-MAX antibody [73C5a] (ab53570)Anti-MAX antibody [73C5a] (ab53570) + immunised recombinant protein
Predicted band size: 18 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted? -
![Flow Cytometry - Anti-MAX antibody [73C5a] (ab53570)](https://www.abcam.com/ps/products/53/ab53570/Images/ab53570-66443-anti-max-antibody-73c5a-flow-cytometry.jpg)
Flow Cytometry - Anti-MAX antibody [73C5a] (ab53570)Overlay histogram showing A549 cells stained with ab53570 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53570, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Datasheets and documents
References (8)
ab53570 has been referenced in 8 publications.
- Mudersbach T et al. Epigenetic control of the angiotensin-converting enzyme in endothelial cells during inflammation. PLoS One 14:e0216218 (2019). PubMed: 31042763
- Lu YC et al. The Molecular Signature of Megakaryocyte-Erythroid Progenitors Reveals a Role for the Cell Cycle in Fate Specification. Cell Rep 25:2083-2093.e4 (2018). PubMed: 30463007
- Studd JB et al. Genetic predisposition to B-cell acute lymphoblastic leukemia at 14q11.2 is mediated by a CEBPE promoter polymorphism. Leukemia N/A:N/A (2018). PubMed: 29977016
- Tikhanovich I et al. Arginine methylation regulates c-Myc-dependent transcription by altering promoter recruitment of the acetyltransferase p300. J Biol Chem 292:13333-13344 (2017). PubMed: 28652407
- Wang M et al. Common genetic variation in ETV6 is associated with colorectal cancer susceptibility. Nat Commun 7:11478 (2016). ChIP ; Human . PubMed: 27145994
- Xue G et al. c-Myc-mediated repression of miR-15-16 in hypoxia is induced by increased HIF-2a and promotes tumor angiogenesis and metastasis by upregulating FGF2. Oncogene N/A:N/A (2014). PubMed: 24704828
- Iliopoulos D et al. Inhibition of miR-193a expression by max and RXRa activates K-Ras and PLAU to mediate distinct aspects of cellular transformation. Cancer Res 71:5144-53 (2011). ChIP ; Human . PubMed: 21670079
- Iliopoulos D et al. STAT3 Activation of miR-21 and miR-181b-1 via PTEN and CYLD Are Part of the Epigenetic Switch Linking Inflammation to Cancer. Mol Cell 39:493-506 (2010). ChIP ; Human . PubMed: 20797623
