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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper
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Anti-MAX antibody [73C5a] (ab53570)

  • Datasheet
Reviews (1) Submit a question References (8)

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Western blot - Anti-MAX antibody [73C5a] (ab53570)
  • Flow Cytometry - Anti-MAX antibody [73C5a] (ab53570)

Key features and details

  • Mouse monoclonal [73C5a] to MAX
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-MAX antibody [73C5a]
    See all MAX primary antibodies
  • Description

    Mouse monoclonal [73C5a] to MAX
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant human MAX

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab53570 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • Clonality

    Monoclonal
  • Clone number

    73C5a
  • Isotype

    IgG2b
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH / Leucine Zipper

Associated products

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Recombinant Protein

    • Recombinant Human MAX protein (ab95309)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab53570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
WB
Recombinant fragment
Application Abreviews Notes
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

WB (1)
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 18 kDa).
Notes
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

WB
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 18 kDa).

Target

  • Relevance

    MAX is a basic helix-loop-helix, leucine zipper protein that forms heterodimers with MYC and MAD. It is involved in regulating gene expression.
  • Cellular localization

    Nuclear
  • Database links

    • Entrez Gene: 4149 Human
    • Omim: 154950 Human
    • SwissProt: P61244 Human
    • Unigene: 285354 Human
    • Alternative names

      • bHLHd4 antibody
      • bHLHd5 antibody
      • bHLHd6 antibody
      • bHLHd7 antibody
      • bHLHd8 antibody
      • Helix loop helix zipper protein antibody
      • MAX protein antibody
      • MGC10775 antibody
      • MGC11225 antibody
      • MGC18164 antibody
      • MGC34679 antibody
      • MGC36767 antibody
      • MYC associated factor X antibody
      • Myc binding novel HLH/LZ protein antibody
      • Orf 1 antibody
      • Orf1 antibody
      • Protein max antibody
      see all

    Images

    • Western blot - Anti-MAX antibody [73C5a] (ab53570)
      Western blot - Anti-MAX antibody [73C5a] (ab53570)
      Anti-MAX antibody [73C5a] (ab53570) + immunised recombinant protein

      Predicted band size: 18 kDa
      Observed band size: 34 kDa
      why is the actual band size different from the predicted?

    • Flow Cytometry - Anti-MAX antibody [73C5a] (ab53570)
      Flow Cytometry - Anti-MAX antibody [73C5a] (ab53570)
      Overlay histogram showing A549 cells stained with ab53570 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53570, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    Protocols

    • Flow cytometry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (8)

    Publishing research using ab53570? Please let us know so that we can cite the reference in this datasheet.

    ab53570 has been referenced in 8 publications.

    • Mudersbach T  et al. Epigenetic control of the angiotensin-converting enzyme in endothelial cells during inflammation. PLoS One 14:e0216218 (2019). PubMed: 31042763
    • Lu YC  et al. The Molecular Signature of Megakaryocyte-Erythroid Progenitors Reveals a Role for the Cell Cycle in Fate Specification. Cell Rep 25:2083-2093.e4 (2018). PubMed: 30463007
    • Studd JB  et al. Genetic predisposition to B-cell acute lymphoblastic leukemia at 14q11.2 is mediated by a CEBPE promoter polymorphism. Leukemia N/A:N/A (2018). PubMed: 29977016
    • Tikhanovich I  et al. Arginine methylation regulates c-Myc-dependent transcription by altering promoter recruitment of the acetyltransferase p300. J Biol Chem 292:13333-13344 (2017). PubMed: 28652407
    • Wang M  et al. Common genetic variation in ETV6 is associated with colorectal cancer susceptibility. Nat Commun 7:11478 (2016). ChIP ; Human . PubMed: 27145994
    • Xue G  et al. c-Myc-mediated repression of miR-15-16 in hypoxia is induced by increased HIF-2a and promotes tumor angiogenesis and metastasis by upregulating FGF2. Oncogene N/A:N/A (2014). PubMed: 24704828
    • Iliopoulos D  et al. Inhibition of miR-193a expression by max and RXRa activates K-Ras and PLAU to mediate distinct aspects of cellular transformation. Cancer Res 71:5144-53 (2011). ChIP ; Human . PubMed: 21670079
    • Iliopoulos D  et al. STAT3 Activation of miR-21 and miR-181b-1 via PTEN and CYLD Are Part of the Epigenetic Switch Linking Inflammation to Cancer. Mol Cell 39:493-506 (2010). ChIP ; Human . PubMed: 20797623

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Western blot abreview for Anti-MAX antibody [73C5a]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (skin keratinocytes)
    Gel Running Conditions
    Reduced Denaturing (AnyKd from BioRad)
    Loading amount
    40 µg
    Specification
    skin keratinocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Mr. Julien Coutier

    Verified customer

    Submitted Feb 07 2017

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