Key features and details
- Rabbit polyclonal to MAX
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAX antibody
See all MAX primary antibodies
DescriptionRabbit polyclonal to MAX
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Cat, Rhesus monkey
Recombinant fragment within Human MAX (internal sequence). The exact sequence is proprietary.
Database link: P61244
- WB: U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts. IP: HEK-293T whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/100 - 1/500.|
|WB||1/500 - 1/3000. Predicted molecular weight: 18 kDa.|
RelevanceMAX is a basic helix-loop-helix, leucine zipper protein that forms heterodimers with MYC and MAD. It is involved in regulating gene expression.
- bHLHd4 antibody
- bHLHd5 antibody
- bHLHd6 antibody
All lanes : Anti-MAX antibody (ab227298) at 1/500 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 (human brain neuroblast cell line) whole cell extract
Lane 4 : SK-N-AS (human brain neuroblastoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 18 kDa
15% SDS-PAGE gel
MAX was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 2 µg of ab227298. Western blot was performed from the immunoprecipitate using ab227298 at 1/1000 dilution. Anti-Rabbit IgG was used as secondary antibody.
Lane 1: preimmune Rabbit IgG instead of ab227298 in HEK-293T whole cell extract.
Lane 2: ab227298 IP in HEK-293T whole cell extract.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227298 has not yet been referenced specifically in any publications.