Overview

  • Product name

  • Description

    Rabbit polyclonal to MAX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Cat, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human MAX (internal sequence). The exact sequence is proprietary.
    Database link: P61244

  • Positive control

    • WB: U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts. IP: HEK-293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab227298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 18 kDa.

Target

  • Relevance

    MAX is a basic helix-loop-helix, leucine zipper protein that forms heterodimers with MYC and MAD. It is involved in regulating gene expression.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • bHLHd4 antibody
    • bHLHd5 antibody
    • bHLHd6 antibody
    • bHLHd7 antibody
    • bHLHd8 antibody
    • Helix loop helix zipper protein antibody
    • MAX protein antibody
    • MGC10775 antibody
    • MGC11225 antibody
    • MGC18164 antibody
    • MGC34679 antibody
    • MGC36767 antibody
    • MYC associated factor X antibody
    • Myc binding novel HLH/LZ protein antibody
    • Orf 1 antibody
    • Orf1 antibody
    • Protein max antibody
    see all

Images

  • All lanes : Anti-MAX antibody (ab227298) at 1/500 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
    Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
    Lane 3 : IMR32 (human brain neuroblast cell line) whole cell extract
    Lane 4 : SK-N-AS (human brain neuroblastoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 18 kDa



    15% SDS-PAGE gel

  • MAX was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 2 µg of ab227298. Western blot was performed from the immunoprecipitate using ab227298 at 1/1000 dilution. Anti-Rabbit IgG was used as secondary antibody.

    Lane 1: preimmune Rabbit IgG instead of ab227298 in HEK-293T whole cell extract
    Lane 2: ab227298 IP in HEK-293T whole cell extract

References

ab227298 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227298.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up