Product nameAnti-MAX antibody
See all MAX primary antibodies
DescriptionRabbit polyclonal to MAX
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Cat, Rhesus monkey
Recombinant fragment within Human MAX (internal sequence). The exact sequence is proprietary.
Database link: P61244
- WB: U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/100 - 1/500.|
|WB||1/500 - 1/3000. Predicted molecular weight: 18 kDa.|
RelevanceMAX is a basic helix-loop-helix, leucine zipper protein that forms heterodimers with MYC and MAD. It is involved in regulating gene expression.
- bHLHd4 antibody
- bHLHd5 antibody
- bHLHd6 antibody
All lanes : Anti-MAX antibody (ab227298) at 1/500 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 (human brain neuroblast cell line) whole cell extract
Lane 4 : SK-N-AS (human brain neuroblastoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 18 kDa
15% SDS-PAGE gel
MAX was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 2 µg of ab227298. Western blot was performed from the immunoprecipitate using ab227298 at 1/1000 dilution. Anti-Rabbit IgG was used as secondary antibody.
Lane 1: preimmune Rabbit IgG instead of ab227298 in HEK-293T whole cell extract.
Lane 2: ab227298 IP in HEK-293T whole cell extract.
ab227298 has not yet been referenced specifically in any publications.