Key features and details
- Rabbit polyclonal to Maxi Potassium channel beta/KCNMB1
- Suitable for: IHC-P, WB, ICC, ICC/IF
- Reacts with: Mouse, Rat, Rabbit, Horse, Chicken, Dog, Human, Pig
- Isotype: IgG
Product nameAnti-Maxi Potassium channel beta/KCNMB1 antibody
DescriptionRabbit polyclonal to Maxi Potassium channel beta/KCNMB1
Specificityab3587 is specific for the Beta-1 subunit of Maxi K+. It does not detect the beta-2 subunit
Tested applicationsSuitable for: IHC-P, WB, ICC, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Horse, Chicken, Dog, Human, Pig
Predicted to work with: Cow
This product was previously labelled as Maxi Potassium channel beta
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Primary antibody notesPotassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open. Maxi K+ beta is the target for possible therapeutics because of its role in blood flow and blood pressure regulation.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab3587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Can be blocked with Maxi Potassium channel beta/KCNMB1 peptide (ab5023). Detects an ~28 kDa protein representing Maxi K+ beta from mouse myometrial smooth muscle cell extracts.|
|ICC||Use at an assay dependent concentration.|
|ICC/IF||1/100. PubMed: 16116057|
FunctionRegulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Increases the apparent Ca(2+)/voltage sensitivity of the KCNMA1 channel. It also modifies KCNMA1 channel kinetics and alters its pharmacological properties. It slows down the activation and the deactivation kinetics of the channel. Acts as a negative regulator of smooth muscle contraction by enhancing the calcium sensitivity to KCNMA1. Its presence is also a requirement for internal binding of the KCNMA1 channel opener dehydrosoyasaponin I (DHS-1) triterpene glycoside and for external binding of the agonist hormone 17-beta-estradiol (E2). Increases the binding activity of charybdotoxin (CTX) toxin to KCNMA1 peptide blocker by increasing the CTX association rate and decreasing the dissociation rate.
Tissue specificityAbundantly expressed in smooth muscle. Low levels of expression in most other tissues. Within the brain, relatively high levels found in hippocampus and corpus callosum.
Sequence similaritiesBelongs to the KCNMB (TC 8.A.14.1) family. KCNMB1 subfamily.
- Information by UniProt
- BK channel beta subunit antibody
- BK channel subunit beta 1 antibody
- BK channel subunit beta-1 antibody
ab3587 at 1/500 dilution staining mouse and chicken cerebellum by Western blot. Membrane enriched protein fraction from liver and cerebellum were separated in a NuPage 4-12% Bis-Tris gel and transferred to a PVDF membrane. The blot was blocked with PIERCE blocking buffer and incubated overnight at 4C with the antibody 1/500 diluted in PIERCE blocking buffer. A HRP secondary antibody was used at a dilution 1/10000 at room temperature for 1hr. ECL plus Western Blotting detection was used as a substrate. ab3587 reacts with the chicken antigen, although the protein seems slightly smaller. It has been reported that b-BK migrates as a 31 kDa band, although the predicted size is 22 kDa
ab3587 has been referenced in 14 publications.
- Nystoriak MA et al. Ser1928 phosphorylation by PKA stimulates the L-type Ca2+ channel CaV1.2 and vasoconstriction during acute hyperglycemia and diabetes. Sci Signal 10:N/A (2017). PubMed: 28119464
- Nieves-Cintrón M et al. Impaired BKCachannel function in native vascular smooth muscle from humans with type 2 diabetes. Sci Rep 7:14058 (2017). PubMed: 29070899
- Wang Y et al. The Involvement of Ca(2+) Signal Pathways in Distal Colonic Myocytes in a Rat Model of Dextran Sulfate Sodium-induced Colitis. Chin Med J (Engl) 129:1185-92 (2016). WB . PubMed: 27174327
- Leo MD et al. Dynamic regulation of ß1 subunit trafficking controls vascular contractility. Proc Natl Acad Sci U S A 111:2361-6 (2014). PubMed: 24464482
- Nourian Z et al. Large Conductance Ca2+-Activated K+ Channel (BKCa) a-Subunit Splice Variants in Resistance Arteries from Rat Cerebral and Skeletal Muscle Vasculature. PLoS One 9:e98863 (2014). WB ; Rat . PubMed: 24921651