Overview

  • Product name

    Anti-Maxi Potassium channel beta/KCNMB1 antibody
    See all Maxi Potassium channel beta/KCNMB1 primary antibodies
  • Description

    Rabbit polyclonal to Maxi Potassium channel beta/KCNMB1
  • Host species

    Rabbit
  • Specificity

    ab3587 is specific for the Beta-1 subunit of Maxi K+. It does not detect the beta-2 subunit
  • Tested applications

    Suitable for: IHC-P, WB, ICC, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Horse, Chicken, Dog, Human, Pig
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human Maxi Potassium channel beta/KCNMB1 aa 90-103.
    Sequence:

    LYHTEDTRDQNQQC


    (Peptide available as ab5023)

  • General notes

     This product was previously labelled as Maxi Potassium channel beta

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    Potassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open. Maxi K+ beta is the target for possible therapeutics because of its role in blood flow and blood pressure regulation.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200.
WB Use a concentration of 2 µg/ml. Can be blocked with Maxi Potassium channel beta/KCNMB1 peptide (ab5023). Detects an ~28 kDa protein representing Maxi K+ beta from mouse myometrial smooth muscle cell extracts.
ICC Use at an assay dependent concentration.
ICC/IF 1/100. PubMed: 16116057

Target

  • Function

    Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Increases the apparent Ca(2+)/voltage sensitivity of the KCNMA1 channel. It also modifies KCNMA1 channel kinetics and alters its pharmacological properties. It slows down the activation and the deactivation kinetics of the channel. Acts as a negative regulator of smooth muscle contraction by enhancing the calcium sensitivity to KCNMA1. Its presence is also a requirement for internal binding of the KCNMA1 channel opener dehydrosoyasaponin I (DHS-1) triterpene glycoside and for external binding of the agonist hormone 17-beta-estradiol (E2). Increases the binding activity of charybdotoxin (CTX) toxin to KCNMA1 peptide blocker by increasing the CTX association rate and decreasing the dissociation rate.
  • Tissue specificity

    Abundantly expressed in smooth muscle. Low levels of expression in most other tissues. Within the brain, relatively high levels found in hippocampus and corpus callosum.
  • Sequence similarities

    Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB1 subfamily.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • BK channel beta subunit antibody
    • BK channel subunit beta 1 antibody
    • BK channel subunit beta-1 antibody
    • BKbeta antibody
    • BKbeta1 antibody
    • Calcium activated potassium channel subfamily M subunit beta 1 antibody
    • Calcium activated potassium channel subunit beta 1 antibody
    • Calcium activated potassium channel subunit beta antibody
    • Calcium-activated potassium channel antibody
    • Calcium-activated potassium channel subunit beta antibody
    • Calcium-activated potassium channel subunit beta-1 antibody
    • Charybdotoxin receptor subunit beta 1 antibody
    • Charybdotoxin receptor subunit beta-1 antibody
    • Hbeta1 antibody
    • hslo beta antibody
    • K(VCA)beta 1 antibody
    • K(VCA)beta antibody
    • K(VCA)beta-1 antibody
    • KCMB1_HUMAN antibody
    • KCNMB 1 antibody
    • Kcnmb1 antibody
    • Large conductance Ca2+ activated K+ channel beta 1 subunit antibody
    • Maxi K channel beta subunit antibody
    • Maxi K channel subunit beta 1 antibody
    • Maxi K channel subunit beta-1 antibody
    • Potassium large conductance calcium activated channel subfamily M beta member 1 antibody
    • Slo beta 1 antibody
    • SLO beta antibody
    • Slo-beta antibody
    • Slo-beta-1 antibody
    • subfamily M subunit beta-1 antibody
    see all

Images

  • ab3587 at 1/500 dilution staining mouse and chicken cerebellum by Western blot. Membrane enriched protein fraction from liver and cerebellum were separated in a NuPage 4-12% Bis-Tris gel and transferred to a PVDF membrane. The blot was blocked with PIERCE blocking buffer and incubated overnight at 4C with the antibody 1/500 diluted in PIERCE blocking buffer. A HRP secondary antibody was used at a dilution 1/10000 at room temperature for 1hr. ECL plus Western Blotting detection was used as a substrate. ab3587 reacts with the chicken antigen, although the protein seems slightly smaller. It has been reported that b-BK migrates as a 31 kDa band, although the predicted size is 22 kDa

    See Abreview

References

This product has been referenced in:

  • Nystoriak MA  et al. Ser1928 phosphorylation by PKA stimulates the L-type Ca2+ channel CaV1.2 and vasoconstriction during acute hyperglycemia and diabetes. Sci Signal 10:N/A (2017). Read more (PubMed: 28119464) »
  • Nieves-Cintrón M  et al. Impaired BKCachannel function in native vascular smooth muscle from humans with type 2 diabetes. Sci Rep 7:14058 (2017). Read more (PubMed: 29070899) »
See all 14 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (Cartilage, articular)
Specification
Cartilage, articular
Fixative
Neutral buffered formalin
Antigen retrieval step
None
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Dr. Ali Mobasheri

Verified customer

Submitted Feb 01 2011

Application
Western blot
Sample
Horse Cell lysate - whole cell (Chondrocytes isolated from equine cartilage)
Loading amount
25 µg
Specification
Chondrocytes isolated from equine cartilage
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Ali Mobasheri

Verified customer

Submitted Jan 20 2011

Application
Western blot
Sample
Chicken Tissue lysate - other (cerebellum)
Loading amount
22 µg
Specification
cerebellum
Blocking step
Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100%

Dr. Soledad Miranda-Rottmann

Verified customer

Submitted Jul 25 2006

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