Product nameAnti-MAZ antibody
See all MAZ primary antibodies
DescriptionRabbit polyclonal to MAZ
Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Pig
Synthetic peptide within residues: FPVFPCTLLA PPFPVLGLDS RGVGGLMNSF PPPQGHAQNP LQVGAELQSR , corresponding to N terminal amino acids 2-51 of Human MAZ (NP_002374)
- 293T cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab83397 is purified by a peptide affinity chromatography method.
Our Abpromise guarantee covers the use of ab83397 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
|IHC-P||Use at an assay dependent concentration. PubMed: 22415301|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionMay function as a transcription factor with dual roles in transcription initiation and termination. Binds to two sites, ME1a1 and ME1a2, within the MYC promoter having greater affinity for the former. Also binds to multiple G/C-rich sites within the promoter of the Sp1 family of transcription factors.
Tissue specificityPresent in kidney, liver and brain. In the brain, highest levels are found in motor cortex and midfrontal cortex (at protein level).
Sequence similaritiesContains 6 C2H2-type zinc fingers.
Cellular localizationNucleus. In brains of Alzheimer disease patients, present in a plaque-like structures.
- Information by UniProt
- MAZ antibody
- MAZ_HUMAN antibody
- MAZI antibody
Anti-MAZ antibody (ab83397) at 1 µg/ml + 293T cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Additional bands at: 85 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling MAZ with ab83397 at 10µg/ml.
This product has been referenced in:
- Franz H et al. The histone code reader SPIN1 controls RET signaling in liposarcoma. Oncotarget 6:4773-89 (2015). Read more (PubMed: 25749382) »
- Smits M et al. Myc-associated zinc finger protein (MAZ) is regulated by miR-125b and mediates VEGF-induced angiogenesis in glioblastoma. FASEB J 26:2639-47 (2012). IHC-P ; Human . Read more (PubMed: 22415301) »