Key features and details
- Rabbit polyclonal to MAZ
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAZ antibody
See all MAZ primary antibodies
DescriptionRabbit polyclonal to MAZ
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Pig, Chimpanzee, Rhesus monkey, Chinese hamster
Synthetic peptide, corresponding to a region within amino acids 427-477 of Human MAZ, BAA33064.1
- HeLa whole cell lysate (ab150035)
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85725 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 49 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay function as a transcription factor with dual roles in transcription initiation and termination. Binds to two sites, ME1a1 and ME1a2, within the MYC promoter having greater affinity for the former. Also binds to multiple G/C-rich sites within the promoter of the Sp1 family of transcription factors.
Tissue specificityPresent in kidney, liver and brain. In the brain, highest levels are found in motor cortex and midfrontal cortex (at protein level).
Sequence similaritiesContains 6 C2H2-type zinc fingers.
Cellular localizationNucleus. In brains of Alzheimer disease patients, present in a plaque-like structures.
- Information by UniProt
- MAZ antibody
- MAZ_HUMAN antibody
- MAZI antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling MAZ with ab85725 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-MAZ antibody (ab85725) at 0.1 µg/ml
Lane 1 : HeLa cells lysate at 50 µg
Lane 2 : HeLa cells lysate at 15 µg
Lane 3 : HeLa cells lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa, 39 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Detection of Human MAZ by Immunoprecipitation in HeLa cell lysate (1 mg for IP, 20% of IP loaded) using ab85725 at 3 ug/mg lysate from HeLa cells (lane 1). Lane 2 is Control IgG IP. Subsequent WB detection was performed using 1 ug/ml ab85725. Detection: Chemiluminescence with exposure times of 30 seconds.
ab85725 has been referenced in 1 publication.
- Castro IC et al. MYC-induced epigenetic activation of GATA4 in lung adenocarcinoma. Mol Cancer Res 11:161-72 (2013). Human . PubMed: 23239811