Overview

  • Product name

  • Description

    Rabbit polyclonal to MAZ
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Pig, Chimpanzee, Rhesus monkey, Chinese hamster
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 427-477 of Human MAZ, BAA33064.1

  • Positive control

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May function as a transcription factor with dual roles in transcription initiation and termination. Binds to two sites, ME1a1 and ME1a2, within the MYC promoter having greater affinity for the former. Also binds to multiple G/C-rich sites within the promoter of the Sp1 family of transcription factors.
  • Tissue specificity

    Present in kidney, liver and brain. In the brain, highest levels are found in motor cortex and midfrontal cortex (at protein level).
  • Sequence similarities

    Contains 6 C2H2-type zinc fingers.
  • Cellular localization

    Nucleus. In brains of Alzheimer disease patients, present in a plaque-like structures.
  • Information by UniProt
  • Database links

  • Alternative names

    • MAZ antibody
    • MAZ_HUMAN antibody
    • MAZI antibody
    • MYC associated zinc finger protein antibody
    • Myc-associated zinc finger protein antibody
    • Pur-1 antibody
    • Pur1 antibody
    • Purine binding transcription factor antibody
    • Purine-binding transcription factor antibody
    • SAF 1 antibody
    • SAF 2 antibody
    • SAF-1 antibody
    • SAF-2 antibody
    • SAF-3 antibody
    • Serum amyloid A activating factor 1 antibody
    • Serum amyloid A activating factor 2 antibody
    • Transcription factor Zif87 antibody
    • ZF87 antibody
    • Zif87 antibody
    • Zinc finger protein 801 antibody
    • Zinc finger protein, 87 kilodaltons antibody
    • ZNF801 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling MAZ with ab85725 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-MAZ antibody (ab85725) at 0.1 µg/ml

    Lane 1 : HeLa cells lysate at 50 µg
    Lane 2 : HeLa cells lysate at 15 µg
    Lane 3 : HeLa cells lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 49 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 35 kDa, 39 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds
  • Detection of Human MAZ by Immunoprecipitation in HeLa cell lysate (1 mg for IP, 20% of IP loaded) using ab85725 at 3 ug/mg lysate from HeLa cells (lane 1). Lane 2 is Control IgG IP. Subsequent WB detection was performed using 1 ug/ml ab85725. Detection: Chemiluminescence with exposure times of 30 seconds.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab85725.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up