• Product name
    Anti-MBD1 antibody [EPR3564]
    See all MBD1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3564] to MBD1
  • Host species
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MBD1 (C terminal). The exact sequence is proprietary.

  • Positive control
    • HeLa, A431, 293T and Jurkat cell lysates; Human tonsil tissue; MCF7 cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab108510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 67 kDa).
IP 1/20.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
    • Tissue specificity
      Widely expressed.
    • Sequence similarities
      Contains 3 CXXC-type zinc fingers.
      Contains 1 MBD (methyl-CpG-binding) domain.
    • Domain
      The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.
      The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides.
      The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.
    • Post-translational
      Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus speckle. Chromosome. Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • CXXC 3 antibody
      • CXXC-type zinc finger protein 3 antibody
      • CXXC3 antibody
      • MBD 1 antibody
      • Mbd1 antibody
      • MBD1_HUMAN antibody
      • MECP1 COMPLEX antibody
      • Methyl CpG binding domain protein 1 antibody
      • Methyl CpG binding domain protein 1 isoform PCM1 antibody
      • Methyl CpG binding protein MBD1 antibody
      • Methyl CpG binding protein splice variant 1 antibody
      • Methyl CpG binding protein splice variant 2 antibody
      • Methyl CpG binding protein splice variant 3 antibody
      • Methyl CpG binding protein splice variant 4 antibody
      • Methyl-CpG-binding domain protein 1 antibody
      • Methyl-CpG-binding protein MBD1 antibody
      • PCM 1 antibody
      • PCM1 antibody
      • Protein containing methyl-CpG-binding domain 1 antibody
      • Regulator of fibroblast growth factor 2 (FGF 2) transcription antibody
      • RFT antibody
      • The regulator of fibroblast growth factor 2(FGF 2) transcription antibody
      see all


    • All lanes : Anti-MBD1 antibody [EPR3564] (ab108510) at 1/500 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 67 kDa

    • ab108510, at 1/100 dilution, staining MBD1 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
    • ab108510, at 1/100 dilution, staining MBD1 in MCF7 cells by Immunofluorescence.


    This product has been referenced in:
    • Hata K  et al. DNA methylation and methyl-binding proteins control differential gene expression in distinct cortical areas of macaque monkey. J Neurosci 33:19704-14 (2013). Macaque monkey . Read more (PubMed: 24336734) »

    See 1 Publication for this product

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