Recombinant
RabMAb

Recombinant Anti-MBD1 antibody [EPR3564] - BSA and Azide free (ab247676)

Submit a review Submit a question

Overview

  • Product name

    Anti-MBD1 antibody [EPR3564] - BSA and Azide free
    See all MBD1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3564] to MBD1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human MBD1 (C terminal). The exact sequence is proprietary.

  • General notes

    ab247676 is the carrier-free version of ab108510 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247676 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: PBS
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR3564

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab247676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.

 
WB Use at an assay dependent concentration. Detects a band of approximately 80 kDa (predicted molecular weight: 67 kDa).
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.

    • Tissue specificity

      Widely expressed.

    • Sequence similarities

      Contains 3 CXXC-type zinc fingers.
      Contains 1 MBD (methyl-CpG-binding) domain.

    • Domain

      The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.
      The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides.
      The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.

    • Post-translational
      modifications

      Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.
      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Nucleus speckle. Chromosome. Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes.
    • Information by UniProt

    • Database links

      • Alternative names

        • CXXC 3 antibody
        • CXXC-type zinc finger protein 3 antibody
        • CXXC3 antibody
        • MBD 1 antibody
        • Mbd1 antibody
        • MBD1_HUMAN antibody
        • MECP1 COMPLEX antibody
        • Methyl CpG binding domain protein 1 antibody
        • Methyl CpG binding domain protein 1 isoform PCM1 antibody
        • Methyl CpG binding protein MBD1 antibody
        • Methyl CpG binding protein splice variant 1 antibody
        • Methyl CpG binding protein splice variant 2 antibody
        • Methyl CpG binding protein splice variant 3 antibody
        • Methyl CpG binding protein splice variant 4 antibody
        • Methyl-CpG-binding domain protein 1 antibody
        • Methyl-CpG-binding protein MBD1 antibody
        • PCM 1 antibody
        • PCM1 antibody
        • Protein containing methyl-CpG-binding domain 1 antibody
        • Regulator of fibroblast growth factor 2 (FGF 2) transcription antibody
        • RFT antibody
        • The regulator of fibroblast growth factor 2(FGF 2) transcription antibody
        see all

      References

      ab247676 has not yet been referenced specifically in any publications.

      Publishing research using ab247676? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab247676.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com