Product nameMBD1 overexpression 293T lysate (whole cell)
DescriptionMBD1 overexpression lysate
ab94263 is a 293T cell transfected lysate in which Human MBD1 has been transiently over-expressed using a pCMV-MBD1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
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BackgroundFunction: Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters. Tissue specificity: Widely expressed. Similarity: Contains 3 CXXC-type zinc fingers. Contains 1 MBD (methyl-CpG-binding) domain. Domain: The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions. The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides. The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions. PTM: Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP. Phosphorylated upon DNA damage, probably by ATM or ATR.
Our Abpromise guarantee covers the use of ab94263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94263 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-MBD1 antibody (ab89534) at 1/500 dilution
Lane 1 :
MBD1 overexpression 293T lysate (whole cell) (ab94263)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94263 has not yet been referenced specifically in any publications.