Overview

Description

  • Nature
    Synthetic

Associated products

Specifications

Our Abpromise guarantee covers the use of ab4918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking

  • Form
    Liquid
  • Additional notes


    This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and human MBD 1, catalog ab2846. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (~ 70-fold) for competitive inhibition of antibody-protein binding reactions.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

General Info

  • Alternative names
    • CXXC 3
    • CXXC-type zinc finger protein 3
    • CXXC3
    • MBD 1
    • Mbd1
    • MBD1_HUMAN
    • MECP1 COMPLEX
    • Methyl CpG binding domain protein 1
    • Methyl CpG binding domain protein 1 isoform PCM1
    • Methyl CpG binding protein MBD1
    • Methyl CpG binding protein splice variant 1
    • Methyl CpG binding protein splice variant 2
    • Methyl CpG binding protein splice variant 3
    • Methyl CpG binding protein splice variant 4
    • Methyl-CpG-binding domain protein 1
    • Methyl-CpG-binding protein MBD1
    • PCM 1
    • PCM1
    • Protein containing methyl-CpG-binding domain 1
    • Regulator of fibroblast growth factor 2 (FGF 2) transcription
    • RFT
    • The regulator of fibroblast growth factor 2(FGF 2) transcription
    see all
  • Function
    Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Contains 3 CXXC-type zinc fingers.
    Contains 1 MBD (methyl-CpG-binding) domain.
  • Domain
    The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.
    The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides.
    The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.
  • Post-translational
    modifications
    Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus speckle. Chromosome. Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes.
  • Information by UniProt

References

This product has been referenced in:
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for contacting us.

The binding with methylated DNA is related to the peptide ab4918.Please chek this is not a prtoein it is a blocking peptide whihc was used to producty antibody ab2846. This is not a fucntional peptide.

The other proteins are full length (ab152513) is full length protein whih chas been tested in WB, SDS-PAGE and ELISA. These applicaiotn doe snot involve any kind of binding with DNA so I am sorry we will not be able to comment on the binding specificity of this protein.

We unfortunaltely do not have Biotin conjugated proteins avialable.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

The peptide is dissolved in water without any salts. For storage in your lab, just aliquot.

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Answer

Thank you for your inquiry.

MBD1 :

I would like to point out that ab4918 is 13 amino acid synthetic peptide whose sequences are derived from human MBD1 protein, C-R(593)SKDLKKPGARK(605) (amino to carboxyl terminus). This peptide can be used with studies using ab2846, but not with other antibodies since it is only a very short peptide.

ab71007 and ab89534 (both anti MBD1 antibodies cannot be used with ab4918. They will not bind this specific sequence.

Both these antibodies will work with a full length protein. Unfortunately, we do not currently offer this in our catalog.

MBD4 :

ab126003 is a recombinant fragment of Human MBD4 (amino acids 68-300) with a His-DHFR tag.
Please be aware thatab84754 is not compatible with ab126003.

I am happy to confirm that ab124460 (https://www.abcam.com/index.html?datasheet=124460 (or use the following: https://www.abcam.com/index.html?datasheet=124460).)will bind ab126003 as it is the immunogen sequence.

Butab124460 is not tested for ELISA. I could offer your customer to take part in the testing discount program for this antibody in ELISA.

I am sorry that I did not have a 100% positive answer for your customer and hope this information is nevertheless helpful.

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Answer

Thanks for sending the link. Unfortunately we don't have a subscription so I was not able to open the paper. Do you have a PDF version of the paper?

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Answer

Thank you for your enquiry. ab4918 is not a functional peptide of any kind. It is just a short peptide used as the immunogen to produce Anti-MBD1 antibody - ChIP Grade (ab2846). So it will not bind 5-methyl cytosine on DNA. I hope this is helpful. Please contact me again if you have any further questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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