Overview

  • Product name

  • Description

    Rabbit polyclonal to MBD2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human MBD2 aa 361-411. The exact sequence is proprietary. (NP_003918.1).
    Database link: Q9UBB5

  • Positive control

    • IHC-P: Human prostate carcinoma tissue. WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab224827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 43 kDa.

Target

  • Function

    Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.
  • Tissue specificity

    Highly expressed in brain, heart, kidney, stomach, testis and placenta.
  • Sequence similarities

    Contains 1 MBD (methyl-CpG-binding) domain.
  • Cellular localization

    Nucleus. Nuclear, in discrete foci. Detected at replication foci in late S phase.
  • Information by UniProt
  • Database links

  • Alternative names

    • Demethylase antibody
    • DMTase antibody
    • MBD 2 antibody
    • Mbd2 antibody
    • MBD2_HUMAN antibody
    • MBD2a antibody
    • Methyl CpG binding domain protein 2 antibody
    • Methyl CpG binding protein MBD2 antibody
    • Methyl-CpG-binding domain protein 2 antibody
    • Methyl-CpG-binding protein MBD2 antibody
    • NY CO 41 antibody
    see all

Images

  • All lanes : Anti-MBD2 antibody (ab224827) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 43 kDa


    Exposure time: 10 seconds
  • Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for MBD2 using ab224827 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.

  • MBD2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab224827 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224827 at 1 µg/ml.

    Lane 1: ab224827 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.


    Detection:  Chemiluminescence with exposure time of 3 seconds.

References

ab224827 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab224827.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up