Key features and details
- Rabbit polyclonal to MBD2
- Suitable for: IHC-P, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MBD2 antibody
See all MBD2 primary antibodies
DescriptionRabbit polyclonal to MBD2
Tested applicationsSuitable for: IHC-P, IP, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human MBD2 aa 361-411. The exact sequence is proprietary. (NP_003918.1).
Database link: Q9UBB5
- IHC-P: Human prostate carcinoma tissue. WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 43 kDa.|
FunctionBinds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.
Tissue specificityHighly expressed in brain, heart, kidney, stomach, testis and placenta.
Sequence similaritiesContains 1 MBD (methyl-CpG-binding) domain.
Cellular localizationNucleus. Nuclear, in discrete foci. Detected at replication foci in late S phase.
- Information by UniProt
- Demethylase antibody
- DMTase antibody
- MBD 2 antibody
All lanes : Anti-MBD2 antibody (ab224827) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 43 kDa
Exposure time: 10 seconds
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for MBD2 using ab224827 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
MBD2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab224827 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224827 at 1 µg/ml.
Lane 1: ab224827 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224827 has not yet been referenced specifically in any publications.