Immunocytochemistry/ Immunofluorescence abreview for Anti-MBD2 antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Liver, cultured cell line)
Specification
Liver, cultured cell line
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Other product details

Dilution
1/50
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS with 1% BSA

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 568 goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Dilution
1/400

Additional data

Additional Notes
Primary Antibody incubation under 25oC for an hour and under 4C for rest of the time of overnight.

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Submitted Feb 06 2009

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