ChIP abreview for Anti-MBD2 antibody

Poor
Abreviews
Application
ChIP
Sample
Rat Cell lysate - nuclear (Rat Neuronal Cortex)
Specification
Rat Neuronal Cortex
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step
Semiquantitative PCR
Positive control
Mecp2 antibody.
Negative control
purified rabbit IgG

Other product details

Dilution
1/50
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: IP dilution buffer +proteinase inhibitor

Additional data

Abcam response
Thanks for your honest feedback. To our knowledge this is the first instance in which ab38646 has been tested in ChIP. It may be that this antibody is not useful for ChIP or perhaps further optimization of ChIP conditions will yet lead to improved ChIP results.

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Submitted Nov 27 2012

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