Western blot abreview for Anti-MBD2 antibody

Excellent
Abreviews
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Sample
Mouse Cell lysate - whole cell (MEL, 293T cell)
Specification
MEL, 293T cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/2500

Secondary antibody

Dilution
1/20000

Detection

Detection method
ECL
Bands
Specific: 45 kDa
Exposure
1 minute(s) and 0 second(s)

Dr. Min Young Kim

Verified customer

Submitted Jun 25 2013

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